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Updated: Jun 16, 2026

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Published on: March 22, 2024
Glucosinolate profiles in wild-growing Lepidium draba L. from Tunisia and Croatia
Wissal Saadellaoui1, Azra Đulović2, Ileana Žeravica2
1Laboratory of Biodiversity, Biotechnology and Climate Change (LR11-ES09), Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia.
Abstract:
The glucosinolate (GSL) profiles in different plant parts of the wild-growing species Lepidium draba L. (syn. Cardaria draba Desv.) (Brassicaceae) from Tunisia and Croatia were determined using UHPLC-DAD-MS/MS. The predominant GSLs in both Tunisian and Croatian samples were glucoraphanin and glucosinalbin, with the leaves and flowers being the richest sources. The study also revealed previously unreported GSLs in L. draba, methionine-derived glucoiberin, glucoalyssin, glucohesperin, and glucolesquerellin, as well as tryptophan-derived glucobrassicin and 4-methoxyglucobrassicin. In addition to the variability of GSL content across different plant parts, this study has also shown the limitations of indirect GSL analysis by isothiocyanates using GC-MS, which can lead to incorrect identification of GSLs due to thermal degradation of the isothiocyanates produced.
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