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Updated: Jul 4, 2026

Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds
Published on: August 21, 2021
Advanced radiation-crosslinked CM-chitosan/gelatin hydrogel for diabetic ulcer treatment with reducing application
Hongwei Li1, Xuefei Chen2, Youbin Fan2
1State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Innovation Platform for Industry-Education Integration in Vaccine Research, School of Public Health, Xiamen University, Fujian 361102, China.
Aims:
The study aims to assess whether a radiation-crosslinked carboxymethyl-chitosan/gelatin hydrogel can exhibit superior healing properties in diabetic wounds through collagen synthesis, epithelial maturation, inflammation regulation and angiogenesis, and determine if it can be applied on alternate days to reduce patient compliance pressure.
Methods:
The study used a full-thickness diabetic wound rat model. The hydrogel was applied either daily or on alternate days. H&E, Masson's trichrome, Sirius red and immunohistochemical staining were employed. Two patients with diabetes were recruited for case studies where the hydrogel was applied on alternate days.
Results:
Under the application of the hydrogel, accelerated healing was observed, with enhanced re-epithelialization and dermal differentiation. The treated groups developed mature skin characteristics absent in the control group, and a well-organized collagen network was observed. There was also accelerated macrophage infiltration, phenotype shift and enhanced angiogenesis at different healing stages. In addition, two patients were positive with alternate-day application of the hydrogel.
Conclusions:
The radiation-crosslinked carboxymethyl-chitosan/gelatin hydrogel shows great potential as a promising modality for diabetic wound management, with both daily and alternate-day applications having immunomodulatory and pro-angiogenic functions.
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