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Updated: May 21, 2025

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

Published on: May 27, 2015

Nanoparticles may influence mast cells gene expression profiles without affecting their degranulation function.

Hannah S Newton¹, Edward Cedrone¹, Jason Grunberger²

¹Nanotechnology Characterization Lab., Cancer Research Technology Program, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD 21702, USA.

Nanomedicine : Nanotechnology, Biology, and Medicine

|April 4, 2025

Summary

This summary is machine-generated.

Most tested nanoparticles and nanomedicines do not affect mast cell degranulation, indicating they are unlikely to worsen allergies. However, some nanomaterials impact immune cells more than functional assays detect.

Keywords:

Degranulation Immunotoxicity Mast cells Nanoparticles non-clinical

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Published on: November 1, 2013

Area of Science:

Nanotechnology
Immunology
Pharmacology

Background:

Mast cells play a crucial role in allergic reactions.
Nanoparticles are increasingly used in medicine, necessitating safety assessments.

Purpose of the Study:

To develop and validate an in vitro method for assessing nanoparticle effects on mast cell degranulation.
To evaluate the impact of clinical-grade nanomedicines and research-grade nanomaterials on IgE-dependent mast cell activation.

Main Methods:

Developed and validated an in vitro assay for IgE-dependent mast cell degranulation.
Tested four clinical-grade nanomedicines (Abraxane, Doxil, AmBisome, Feraheme) and three PAMAM dendrimers.
Utilized single-cell sequencing to identify differentially expressed genes (DEG).

Main Results:

Most tested nanoparticles did not alter mast cell degranulation.
Abraxane and Doxil decreased degranulation; Abraxane also reduced FcεR expression.
Feraheme and amine-terminated dendrimers induced DEG without functional changes in degranulation.

Conclusions:

Nanoparticles and nanomedicines are generally unlikely to exacerbate pre-existing allergies.
Some nanomaterials exert effects on immune cells beyond functional degranulation assays.
Comprehensive assessment of nanomaterial immunotoxicity is essential.