Multi-omics insights into the roles of CCNB1, PLK1, and HPSE in breast cancer progression: implications for prognosis and immunotherapy
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View abstract on PubMed
Summary
This summary is machine-generated.Cyclin B1 (CCNB1), Polo-Like Kinase 1 (PLK1), and Heparanase (HPSE) drive breast cancer growth and metastasis. These genes are potential prognostic biomarkers and immunotherapy targets, with CCNB1 and PLK1 impacting proliferation and HPSE influencing immune response.
Area Of Science
- Oncology
- Molecular Biology
- Immunology
Background
- Cyclin B1 (CCNB1), Polo-Like Kinase 1 (PLK1), and Heparanase (HPSE) are implicated in cancer, but their specific roles in breast cancer progression are not fully understood.
- Investigating these genes is crucial for understanding breast cancer development and identifying therapeutic targets.
Purpose Of The Study
- To elucidate the roles of CCNB1, PLK1, and HPSE in breast cancer progression using a comprehensive multi-omics analysis.
- To identify these genes as potential prognostic biomarkers and therapeutic targets for breast cancer.
Main Methods
- Integrative analysis of transcriptomics, proteomics, DNA methylation, immune infiltration, and single-cell RNA sequencing.
- Functional validation through si-RNA knockdown experiments to assess the impact on cell proliferation.
- Correlation analysis of gene expression and DNA methylation with clinical outcomes and immune cell infiltration.
Main Results
- CCNB1, PLK1, and HPSE are significantly upregulated in breast tumors at both mRNA and protein levels.
- CCNB1 and PLK1 promote tumor growth and metastasis, while HPSE is associated with immune pathways and has immunotherapeutic potential.
- High expression of these genes correlates with poor prognosis, and their knockdown reduces breast cancer cell proliferation, indicating regulatory interactions.
Conclusions
- CCNB1, PLK1, and HPSE are key drivers of breast cancer progression and serve as valuable prognostic biomarkers.
- The involvement of these genes in immune regulation suggests their potential as targets for novel breast cancer immunotherapies.
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