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Related Concept Videos

Resting Membrane Potential01:24

Resting Membrane Potential

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The relative difference in electrical charge, or voltage, between the inside and the outside of a cell membrane, is called the membrane potential. It is generated by differences in permeability of the membrane to various ions and the concentrations of these ions across the membrane.
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The resting membrane potential of a neuron (-70mV) is sustained due to the selective ion permeability of the membrane. At the resting potential, the membrane is slightly permeable to ions like sodium (Na+) and chloride (Cl−) and highly permeable to potassium ions (K+). Differences in the ions' concentration inside the cell compared to the outside are maintained by membrane transport proteins like channels and pumps.
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Updated: May 16, 2025

Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats
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Long-term spontaneous membrane currents in DRG neurons.

Sodikdjon A Kodirov1,2,3,4, Vera B Plakhova1, Owen P Hamill5

  • 1Pavlov Institute of Physiology, Russian Academy of Sciences, Saint Petersburg, Russia.

Journal of Receptor and Signal Transduction Research
|April 5, 2025
PubMed
Summary

Researchers observed novel spontaneous inward currents in rat dorsal root ganglion (DRG) neurons, resembling purinergic receptor activity. This discovery may advance understanding of pain mechanisms.

Keywords:
Ion channelsnonsynaptic neurotransmissionpatch-clamp

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Area of Science:

  • Neuroscience
  • Electrophysiology

Background:

  • Voltage-dependent sodium (Nav) channels in dorsal root ganglion (DRG) neurons are crucial for pain signaling.
  • Standard patch-clamp electrophysiology is used to study these currents.

Purpose of the Study:

  • To investigate novel spontaneous activities in DRG neurons during whole-cell patch-clamp recordings.
  • To characterize the nature and potential implications of these observed currents.

Main Methods:

  • Whole-cell patch-clamp electrophysiology on freshly isolated neonatal rat DRG neurons.
  • Recording of voltage-dependent sodium currents (INa).

Main Results:

  • Spontaneous inward currents with fast rise and heterogeneous decay phases were observed in a subset of DRG neurons.
  • The waveform matched responses of P2X purinergic receptors to ATP.
  • This novel activity occurred at -60 mV and was enhanced by hyperpolarization.

Conclusions:

  • Observed spontaneous currents are distinct from previously described membrane potential instabilities in DRG neurons.
  • These novel activities may offer new avenues for understanding and targeting pain mechanisms.
  • Pharmacological characterization of these events could be key to elucidating their role in pain.