Identification and functional analysis of proteins in Anabarilius grahami at different growth stages based on 4D-DIA...

This study compared the proteome of Anabarilius grahami at three growth stages with Carassius auratus at a single growth stage to identify and analyze protein functions in A. grahami using quantitative proteomics technology. A total of 1821 proteins were identified, among which six differentially abundant proteins (DAPs) were found as biomarkers for discriminating C. auratus and A. grahami. Additionally, seven DAPs were identified as factors that could discriminate A. grahami at the three growth stages studied. Furthermore, aspartate aminotransferase and alanine transaminase were identified as key enzymes involved in the synthesis of glutamic acid and aspartic acid, while very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase and CXXC motif containing zinc-binding protein were associated with the synthesis of polyunsaturated fatty acids. These findings provide deeper insights into the nutritional and functional properties of proteins in A. grahami, offering theoretical significance for the utilization of its protein resources and the development of A. grahami related products.