Synthesis, characterization, and bioactivity of selenium nanoparticles stabilized by regenerated chitin nanofibers

Selenium nanoparticles (SeNPs) have garnered significant attention for their advantageous biological properties and low toxicity. However, their practical application has been constrained by limited stability. In this study, regenerated chitin nanofibers (Re-ChNFs) were utilized to improve the stability and dispersion of SeNPs through a redox reaction involving ascorbic acid and sodium selenite. The findings revealed that the SeNPs were effectively adsorbed onto the surface of the Re-ChNFs, resulting in a uniform size and distribution that facilitated the formation of amorphous, zero-valent Re-ChNFs-stabilized SeNPs (Re-ChNFs/SeNPs). The selenium concentration within the Re-ChNFs/SeNPs was determined to be 121.60 mg/L. And the synthesized Re-ChNFs/SeNPs displayed a notably heightened capacity for scavenging DPPH, ABTS, hydroxyl radicals, and superoxide anion radicals in comparison to Re-ChNFs and SeNPs alone. Moreover, in vitro assays demonstrated that Re-ChNFs/SeNPs effectively suppressed the proliferation of HepG2 and HCT116 cancer cells in a concentration-dependent manner. This suggests that Re-ChNFs/SeNPs hold potential as an antioxidant or anticancer therapeutic agents, with promising applications in the fields of nutrition and healthcare.