Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Improving Translational Accuracy02:07

Improving Translational Accuracy

Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
Improving Translational Accuracy02:07

Improving Translational Accuracy

Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
Data Validation01:03

Data Validation

Data validation is an essential part of a comprehensive assessment. Validation is confirming or verifying and opening the door to gathering more assessment data as it clarifies vague or unclear data. The process of checking and verifying the collected information is called data validation. The primary purpose of data validation is to ensure data is as free from error, bias, and misinterpretation as possible.
Nursing assessment guides are generally based on holistic models rather than medical...
Targets for Drug Action: Overview01:26

Targets for Drug Action: Overview

Drugs target macromolecules to modify ongoing cellular processes. Primary drug targets include receptors, ion channels, transporters, and enzymes.
Receptors are either membrane-spanning or intracellular proteins, which upon binding a ligand, get activated and transmit the signal downstream to elicit a response. Drugs bind receptors, either mimicking the action of endogenous ligands or blocking the receptor activity to bring about a modified response. Nearly 35% of approved drugs target the G...
Data Validation01:15

Data Validation

Method validation is a crucial process in analytical chemistry designed to confirm that a given method consistently produces reliable and high-quality results. This process is essential when a method is applied to different sample matrices or when procedural modifications are made, ensuring that the results meet acceptable standards across various applications.
Key parameters for method validation include:
In Vitro Drug Release Testing: Overview, Development and Validation01:10

In Vitro Drug Release Testing: Overview, Development and Validation

In vitro dissolution and drug release tests assess how quickly and how much of a drug is released from its dosage form into an aqueous medium under standardized laboratory conditions. These tests are essential tools in pharmaceutical development and quality assurance, offering insight into the drug's performance before clinical use.During formulation development, dissolution testing identifies incomplete or inconsistent drug release issues. It also supports decisions on selecting the optimal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNA G-Quadruplex RIBOTAC-Mediated Targeted Degradation of lncRNA TERRA.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Development of PROTAC-Based Strategies for Cannabinoid Receptor Type 1 (CB<sub>1</sub>R) Degradation in Cancer.

ACS pharmacology & translational science·2025
Same author

Druglike Molecular Degraders of the Oncogenic RNA-Binding Protein HuR.

JACS Au·2025
Same author

PROTAC and Molecular Glue Degraders of the Oncogenic RNA Binding Protein Lin28.

Macromolecular bioscience·2024
Same author

Targeting MicroRNAs with Small Molecules.

Non-coding RNA·2024
Same author

Targeted Degradation of Structured RNAs via Ribonuclease-Targeting Chimeras (RiboTacs).

Expert opinion on drug discovery·2023
Same journal

From mitochondrial signal to discovery decision: a reserve-demand framework for translational Risk assessment.

Expert opinion on drug discovery·2026
Same journal

Advances in 3D cell culture models for alzheimer's disease drug discovery.

Expert opinion on drug discovery·2026
Same journal

Are patient-derived models of amyotrophic lateral sclerosis a game changer for novel drug discovery?

Expert opinion on drug discovery·2026
Same journal

Unfolding prion misfolding and the challenge of identifying effective therapeutics: is there hope on the horizon?

Expert opinion on drug discovery·2026
Same journal

Balancing optimism versus potential risks of AI-driven drug discovery.

Expert opinion on drug discovery·2026
Same journal

Trustworthy and explainable AI for drug discovery.

Expert opinion on drug discovery·2026
See all related articles

Related Experiment Video

Updated: Jun 15, 2026

Chemical Inactivation of the E3 Ubiquitin Ligase Cereblon by Pomalidomide-based Homo-PROTACs
10:44

Chemical Inactivation of the E3 Ubiquitin Ligase Cereblon by Pomalidomide-based Homo-PROTACs

Published on: May 15, 2019

13.1K

Advancing target validation with PROTAC technology.

M Leora Spitz1, Aseel Kashkush1, Raphael I Benhamou1

  • 1The Institute for Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University, Jerusalem, Israel.

Expert Opinion on Drug Discovery
|April 6, 2025
PubMed
Summary
This summary is machine-generated.

Targeted protein degradation (TPD) using PROteolysis TArgeting Chimeras (PROTACs) offers new drug discovery avenues. Validating PROTAC target engagement and ternary complex formation is crucial for confirming protein degradation and advancing research.

Keywords:
Drug designPROTACsmall moleculestarget validationtargeted degradationternary complexubiquitination

More Related Videos

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
05:33

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

Published on: November 9, 2020

9.3K
Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes
07:22

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

3.2K

Related Experiment Videos

Last Updated: Jun 15, 2026

Chemical Inactivation of the E3 Ubiquitin Ligase Cereblon by Pomalidomide-based Homo-PROTACs
10:44

Chemical Inactivation of the E3 Ubiquitin Ligase Cereblon by Pomalidomide-based Homo-PROTACs

Published on: May 15, 2019

13.1K
High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
05:33

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

Published on: November 9, 2020

9.3K
Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes
07:22

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

3.2K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • Targeted protein degradation (TPD) is an emerging therapeutic strategy.
  • PROteolysis TArgeting Chimeras (PROTACs) are a key TPD technology for degrading disease-associated proteins.
  • Validating PROTAC efficacy requires assessing target engagement and ternary complex formation.

Purpose of the Study:

  • To review methods for validating PROTAC target engagement and ternary complex formation.
  • To discuss the application of PROTACs as chemical probes for target identification.
  • To highlight the importance of target validation in PROTAC-based drug development.

Main Methods:

  • In silico modeling
  • Structural biology techniques
  • In vitro biochemical assays
  • In cellulo experiments
  • Proteomics analyses

Main Results:

  • Various methods exist to validate PROTAC target engagement and ternary complex formation.
  • Proteomics is vital for understanding PROTAC mechanisms and outcomes.
  • PROTACs are increasingly used as chemical probes for target validation.

Conclusions:

  • Thorough target validation, especially confirming ternary complex formation under physiological conditions, is essential for PROTAC development.
  • PROTAC technology holds significant promise for drug discovery and biological research.
  • Continued exploration of PROTACs will advance both chemical and biological sciences.