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Comparative analysis of MALDI-TOF MS and RAPD for grouping Streptococcus agalactiae isolated from subclinical

Ligia Beatriz Rizzanti Pereira1, Breno Luis Nery Garcia1, Carlos Eduardo Fidelis1

  • 1Department of Animal Nutrition and Production, School of Veterinary Medicine and Animal Sciences, University of São Paulo, Pirassununga, 13653-900, SP, Brazil.

Microbial Pathogenesis
|April 6, 2025
PubMed

Insights

Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) effectively grouped Streptococcus agalactiae isolates from mastitis. This method correlated well with antimicrobial susceptibility, offering a rapid diagnostic tool for dairy herds.

Area of Science:

  • Veterinary Microbiology
  • Bacteriology
  • Diagnostic Technology

Background:

  • Streptococcus agalactiae is a contagious pathogen causing subclinical mastitis in dairy animals.
  • Accurate identification and characterization are crucial for managing S. agalactiae outbreaks.
  • Antimicrobial susceptibility profiling aids in treatment decisions for mastitis.

Purpose of the Study:

  • To evaluate Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for grouping S. agalactiae isolates.
  • To associate antimicrobial susceptibility profiles with MALDI-TOF MS groupings.
  • To compare MALDI-TOF MS grouping with Random Amplified Polymorphic DNA (RAPD) clustering.

Main Methods:

  • Collected 198 milk samples from cows and buffaloes across three farms.
  • Performed antimicrobial susceptibility testing for 10 antimicrobials using Minimum Inhibitory Concentration (MIC).
  • Grouped S. agalactiae isolates using MALDI-TOF MS and RAPD analysis.

Main Results:

  • MALDI-TOF MS identified 8 distinct groups, successfully grouping all isolates.
  • RAPD identified 33 clusters, grouping only 100 isolates.
  • Both methods showed isolates from the same herd had similar susceptibility profiles.
  • High sensitivity (>90%) observed for ceftiofur, penicillin, oxacillin, amoxicillin, cefquinome, gentamicin, and cefoxitin.

Conclusions:

  • MALDI-TOF MS is a valuable tool for rapid grouping of S. agalactiae.
  • MALDI-TOF MS grouping aligns with antimicrobial susceptibility patterns.
  • MALDI-TOF MS offers a reliable alternative to RAPD for pathogen grouping in mastitis studies.
Keywords:
Antimicrobial resistanceBovine mastitisMALDI-TOF MSRAPDStreptococcus agalactiae

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