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HPLC-studies on nonmercapt-mercapt conversion of human serum albumin

M Sogami, S Era, S Nagaoka

International Journal of Peptide and Protein Research

|April 1, 1985

Abstract:

Human mercaptalbumin (HMA) and nonmercaptalbumin (HNA) could be separated by high-performance liquid chromatography (HPLC) at neutral pH. Using HPLC, the present authors found the nonmercapt-mercapt conversion (HNA----HMA) during hemodialysis and the mercapt-nonmercapt conversion (HMA----HNA) after hemodialysis in chronic renal failure, indicating HMA as the covalent carrier protein for sulfur-containing amino acids.

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