High performance liquid chromatographic analysis of flavin adenine dinucleotide in whole blood

A method for the determination of Flavin Adenine Dinucleotide in whole blood is presented. The method comprises extraction of the vitamer by using a 6% trichloroacetic acid-20% acetonitrile mixture and direct analysis of the sample extract by using a straight-phase high performance liquid chromatography. A linear calibration curve was obtained for standard vitamer and a satisfactory recovery was observed from spiked samples. Evidence for reproducibility and precision of the assay is presented. The method was applied to determine vitamer levels in blood of healthy subjects.