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Cytoduction Preserves Genetic Diversity Following Plasmid Transfer Into Pooled Yeast Libraries.

Han-Ying Jhuang1, Dimitra Aggeli1, Gregory I Lang1

  • 1Department of Biological Sciences, Lehigh University, Bethlehem, PA, USA.

Yeast (Chichester, England)
|April 7, 2025
PubMed
Summary
This summary is machine-generated.

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Gene expression is stable despite widespread cis and trans regulatory divergence in <i>Saccharomyces</i> yeasts.

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Prevalence, distribution, and mechanisms of genetic dominance in the yeast mating pathway.

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PLoS genetics·2023

Cytoduction efficiently introduces plasmids into diverse yeast populations, preserving genotype frequencies unlike traditional transformation. This method is crucial for genetic engineering applications requiring large, heterogeneous yeast pools.

Area of Science:

  • Yeast genetics
  • Molecular biology
  • Population genetics

Background:

  • Introducing plasmids into yeast is essential for genetic engineering and phenotypic assays.
  • Traditional methods like chemical transformation can alter population structure in large variant pools.

Purpose of the Study:

  • To compare the efficiency of cytoduction versus transformation for introducing plasmids into heterogeneous yeast populations.
  • To quantify the impact of each method on the diversity and frequency of genotypes within the yeast pool.
  • To identify quantitative trait loci (QTL) influencing both cytoduction and transformation efficiencies.

Main Methods:

  • Utilized a highly diverse barcoded yeast collection.
  • Introduced drug-marked plasmids using both transformation and cytoduction (mating without nuclear fusion).
Keywords:
cytoductionplasmidstransformationyeast libraries

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  • Quantified genotype frequencies in initial and final yeast pools to assess method efficiency.

    • Main Results:

    • Cytoduction preserved nearly all initial genotypes in the final pool, maintaining their original frequencies.
    • Transformation significantly altered genotype frequencies, unlike cytoduction.
    • Mapped QTL associated with variations in cytoduction and transformation efficiencies.

    Conclusions:

    • Cytoduction is a highly efficient method for introducing plasmids into diverse yeast populations, preserving genetic diversity.
    • This technique is advantageous for applications such as bulk segregant analysis, deep mutational scanning, and large-scale gene editing.
    • Cytoduction offers a superior alternative to transformation for maintaining population structure in yeast genetic studies.