Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Injection-Site Sarcomas in Rodent Carcinogenicity Studies: Human Relevance and Labeling Implications.

Toxicologic pathology·2026
Same author

<i>Salmonella</i> Typhi asparaginase-dependent activation of GCN2 promotes bacterial killing in murine macrophages.

bioRxiv : the preprint server for biology·2026
Same author

Discovering paracrine regulators of cell type composition from spatial transcriptomics using SPER.

Bioinformatics advances·2026
Same author

Using Statewide Administrative Data and Brief Mental Health Screening to Estimate the Prevalence of Mental Illness Among Probationers.

Probation journal·2026
Same author

Single-molecule validation and optimised protocols for the use of secondary nanobodies in multiplexed immunoassays.

Journal of microscopy·2026
Same author

Impairment in stimulus-response learning as a cognitive biomarker in a model of synucleinopathy.

Translational psychiatry·2026
Same journal

Fundamentals, Measurement and Regulation of the Conductance of Single Molecule Junctions.

Angewandte Chemie (International ed. in English)·2026
Same journal

Quantitative Photoswitching of Spin States in o-Fluoroazobenzene-Loaded Metal-Organic Frameworks.

Angewandte Chemie (International ed. in English)·2026
Same journal

Cobalt Nanoparticles Confined in Defective Carbon Matrices for Robust Intermittent CO<sub>2</sub> Methanation.

Angewandte Chemie (International ed. in English)·2026
Same journal

Copper(II/III) Redox Couple Enables C─H Methylation via a Radical Mechanism Analogous to SAM Enzymes.

Angewandte Chemie (International ed. in English)·2026
Same journal

Ring Strain Engineering of Cyclic Ethers for High-Performance Sodium Metal Batteries.

Angewandte Chemie (International ed. in English)·2026
Same journal

Bond Length as a Unified Descriptor for Stable Iodine Battery.

Angewandte Chemie (International ed. in English)·2026
See all related articles

Related Experiment Video

Updated: May 15, 2025

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
09:12

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

7.8K

A Single-Molecule Liposome Assay for Membrane Permeabilization.

Krzysztof Marek Bąk1, Daniel Edwards1,2, Dylan George1,2

  • 1EaStCHEM School of Chemistry, University of Edinburgh, Joseph Black Building, David Brewster Rd, Edinburgh, EH9 3FJ, UK.

Angewandte Chemie (International Ed. in English)
|April 7, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a sensitive single-molecule assay for rapid screening of compounds that disrupt cell membranes. The high-throughput method aids in discovering new drugs for diseases and antimicrobial applications.

Keywords:
Fluorescence microscopyIon channelsIonophoresMembranesSingle‐molecule studies

More Related Videos

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
07:33

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Published on: December 19, 2020

6.2K
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

11.7K

Related Experiment Videos

Last Updated: May 15, 2025

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
09:12

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

7.8K
Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
07:33

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Published on: December 19, 2020

6.2K
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

11.7K

Area of Science:

  • Biophysics
  • Drug Discovery
  • Microfluidics

Background:

  • Cell membrane disruption is linked to various diseases and antimicrobial drug activity.
  • Rapid screening of membrane-disrupting compounds is crucial for therapeutic drug development.

Purpose of the Study:

  • To develop a highly sensitive assay for studying membrane permeabilization.
  • To enable rapid screening of compounds that disrupt biological membranes.

Main Methods:

  • Integration of single-molecule confocal microscopy with fast-flow microfluidics.
  • Assay uses large unilamellar vesicles (LUVs) with minimal dye molecules.
  • High-throughput detection of ~1000 vesicles per minute without liposome immobilization.

Main Results:

  • Achieved unparalleled sensitivity with detection limits as low as 135 pM.
  • Identified permeabilization by as few as eight molecules per vesicle for active compounds.
  • Demonstrated a robust platform for investigating membrane-disrupting agents.

Conclusions:

  • The developed assay is a powerful tool for identifying novel membrane-disrupting agents.
  • This platform can accelerate the discovery of therapeutics for diseases and antimicrobial applications.
  • The assay's sensitivity is suitable for studying compounds implicated in neurodegenerative diseases.