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High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

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Published on: January 11, 2011

Optical Probes for Cellular Imaging of G-quadruplexes: Beyond Fluorescence Intensity Probes.

Jenna Robinson¹, Aatikah Majid¹, Marina K Kuimova¹

¹Department of Chemistry, Imperial College London Molecular Sciences Research Hub, White City Campus, 82 Wood Lane, London, W12 0BZ, UK.

Angewandte Chemie (International Ed. in English)

|April 7, 2025

Summary

New optical probes enable unbiased detection of G-quadruplex (G4) structures in cells. These probes overcome limitations of intensity-based methods, advancing the study of G4s in biological regulation.

Keywords:

Cellular Imaging Fluorescence Lifetime Phosphorescence Lifetime Quadruplex DNA Ratiometric Probes

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Last Updated: Jul 21, 2026

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Published on: January 11, 2011

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Published on: February 24, 2026

Area of Science:

Biochemistry
Molecular Biology
Genetics

Background:

G-quadruplex (G4) structures in DNA and RNA are increasingly recognized for their roles in biological regulation.
Research has progressed from in vitro studies to genome-wide detection within cellular environments.
Understanding G4s is crucial for fields like cancer research and gene expression regulation.

Purpose of the Study:

To review recent advancements in designing optical probes for G-quadruplex (G4) detection.
To highlight probes that are independent of photoluminescent intensity for unbiased cellular analysis.
To discuss the potential of these probes in advancing G4 biology research.

Main Methods:

Review of intensity-independent optical probe designs for G-quadruplexes.
Discussion of probes utilizing fluorescence or phosphorescence lifetime.
Exploration of spectral ratiometric and single-molecule detection probes.

Main Results:

Intensity-independent probes offer improved, unbiased G4 detection in cellular experiments.
Lifetime-based, ratiometric, and single-molecule probes mitigate issues with probe uptake and concentration.
These advanced probes facilitate more accurate G4 analysis in biological contexts.

Conclusions:

Novel optical probe designs are essential for overcoming limitations in G4 detection.
Intensity-independent modalities significantly enhance the reliability of cellular G4 studies.
Further development is needed to fully exploit these probes for robust G4 biology research.