Dual-Quenching Mechanism-Based Fluorometric Sensing Platform for Precise D-Penicillamine Quantification in...

Area of Science:

• Analytical Chemistry
• Biomedical Engineering
• Materials Science

Background:

• D-penicillamine (D-PA) is vital for treating Wilson's disease, rheumatoid arthritis, and heavy metal poisoning.
• Precise D-PA quantification is essential due to its narrow therapeutic window and toxicity.
• Current detection methods may lack the sensitivity required for effective therapeutic drug monitoring.

Purpose of the Study:

• To develop a novel, highly sensitive fluorometric method for D-penicillamine detection.
• To establish a sophisticated sensing platform for enhanced analytical performance.
• To validate the method for therapeutic drug monitoring in biological samples.

Main Methods:

• Utilized red-emissive carbon dots (R@CDs) and cobalt ions for a novel sensing platform.
• Employed a dual-quenching mechanism involving competitive displacement and inner filter effects.
• Validated the method using rat plasma samples from healthy and diabetic models.

Main Results:

• Achieved excellent linearity (R² = 0.9971) and an ultra-low limit of detection (0.0041 µM).
• Successfully quantified D-PA in rat plasma, demonstrating applicability in therapeutic drug monitoring.
• Revealed variations in D-PA pharmacokinetics between healthy and diabetic rats.

Conclusions:

• The developed fluorometric method offers high sensitivity and reliability for D-PA detection.
• This method has significant potential for personalized therapeutic drug monitoring.
• Findings contribute to a better understanding of D-PA disposition in different physiological states.