Method Development for Determining β-Nicotinamide Mononucleotide (NMN) in Cosmetics Using m-PFC-HPLC.

Area of Science:

• Cosmetic Science
• Analytical Chemistry
• Quality Control

Background:

• Nicotinamide Mononucleotide (NMN) is a popular cosmetic ingredient.
• Lack of a standardized detection method hinders NMN cosmetic quality control.

Purpose of the Study:

• Develop and validate a reliable detection method for NMN.
• Ensure quality control for NMN in cosmetic products like facial masks, emulsions, and creams.

Main Methods:

• Utilized a novel multi-plug filtration clean-up (m-PFC) purification column with multi-walled carbon nanotubes (MWCNTs).
• Established and validated a High-Performance Liquid Chromatography (HPLC) method for NMN detection.
• Verified the method's performance across three cosmetic matrices.

Main Results:

• Achieved good linearity (5.0-500 μg/mL) and a low limit of quantification (5.0 mg/kg).
• Demonstrated high precision (RSD < 3%) and excellent recovery rates (93.9%-109.4%).
• Applied method to commercial products, finding NMN absent in some products claiming its presence.

Conclusions:

• The developed HPLC method is sensitive, accurate, and reliable for NMN detection in cosmetics.
• Provides essential technical support for cosmetic regulatory bodies.
• Highlights the need for established standards and increased supervision for NMN cosmetics.