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Method Development for Determining β-Nicotinamide Mononucleotide (NMN) in Cosmetics Using m-PFC-HPLC.

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A new detection method for Nicotinamide Mononucleotide (NMN) in cosmetics was developed. This reliable HPLC method ensures quality control, revealing some products lack the advertised NMN content.

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Area of Science:

  • Cosmetic Science
  • Analytical Chemistry
  • Quality Control

Background:

  • Nicotinamide Mononucleotide (NMN) is a popular cosmetic ingredient.
  • Lack of a standardized detection method hinders NMN cosmetic quality control.

Purpose of the Study:

  • Develop and validate a reliable detection method for NMN.
  • Ensure quality control for NMN in cosmetic products like facial masks, emulsions, and creams.

Main Methods:

  • Utilized a novel multi-plug filtration clean-up (m-PFC) purification column with multi-walled carbon nanotubes (MWCNTs).
  • Established and validated a High-Performance Liquid Chromatography (HPLC) method for NMN detection.
  • Verified the method's performance across three cosmetic matrices.

Main Results:

  • Achieved good linearity (5.0-500 μg/mL) and a low limit of quantification (5.0 mg/kg).
  • Demonstrated high precision (RSD < 3%) and excellent recovery rates (93.9%-109.4%).
  • Applied method to commercial products, finding NMN absent in some products claiming its presence.

Conclusions:

  • The developed HPLC method is sensitive, accurate, and reliable for NMN detection in cosmetics.
  • Provides essential technical support for cosmetic regulatory bodies.
  • Highlights the need for established standards and increased supervision for NMN cosmetics.