Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Long term oscillation in glycolysis.

J Das, H G Busse

    Journal of Biochemistry
    |March 1, 1985
    PubMed
    Summary

    Researchers extended glycolytic oscillations in yeast extracts by adjusting pH, phosphate, enzyme levels, and temperature. Diluting the extract prolonged the period from 20 minutes to 6 hours.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Towards modelling mental disorders in zebrafish. Neurexins severely modulate anxiety, affiliative social behaviour and aggression.

    Molecular psychiatry·2026
    Same author

    Comparison of PET-CT and contrast-enhanced CT in the evaluation of recurrent oral squamous cell carcinoma.

    International journal of oral and maxillofacial surgery·2026
    Same author

    Multimodal machine learning mobility assessment in Parkinson's disease within supervised and unsupervised settings.

    Journal of neuroengineering and rehabilitation·2026
    Same author

    Multi-modal gait assessment in Parkinson's Disease: A pilot study examining the impact of terrain and environment.

    Gait & posture·2026
    Same author

    Active-reset protein sensors enable continuous in vivo monitoring of inflammation.

    Science (New York, N.Y.)·2024
    Same author

    Field tuning Kitaev systems for spin fractionalization and topological order.

    Journal of physics. Condensed matter : an Institute of Physics journal·2024

    Area of Science:

    • Biochemistry
    • Yeast Metabolism
    • Biochemical Oscillations

    Background:

    • Glycolytic oscillations are fundamental metabolic processes in yeast.
    • Understanding factors influencing oscillation period is crucial for metabolic control.
    • Saccharomyces uvarum exhibits complex glycolytic dynamics.

    Purpose of the Study:

    • To investigate methods for increasing the period of glycolytic oscillations in yeast extracts.
    • To determine the influence of pH, phosphate, enzyme concentrations, and temperature on oscillation duration.

    Main Methods:

    • Systematic variation of pH, phosphate concentration, enzyme concentration, and temperature.
    • Analysis of stable oscillatory transients in Saccharomyces uvarum extracts.
    • Dilution of yeast extract with buffer while maintaining metabolite concentrations.

    Main Results:

    • Stable oscillations were achieved at a pH of approximately 6.5.
    • Increased phosphate concentration, decreased enzyme concentration, and lower temperatures extended the oscillation period.
    • Dilution of the extract successfully prolonged the period from 20 minutes to up to 6 hours.

    Conclusions:

    • The period of glycolytic oscillations in yeast is highly sensitive to environmental and biochemical parameters.
    • Precise control over pH, metabolite, and enzyme levels can significantly extend oscillation duration.
    • Extract dilution offers a viable strategy for achieving prolonged glycolytic oscillations.

    Related Experiment Videos