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Adenovirus-dependent increase in cell membrane permeability.

P Seth, I Pastan, M C Willingham

    The Journal of Biological Chemistry
    |August 15, 1985
    PubMed
    Summary
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    Adenovirus type 2 (Ad2) triggers the release of choline from KB cells, primarily as choline phosphate. This release is dependent on Ad2 concentration, incubation time, and pH, and is inhibited by heat treatment and fetal calf serum.

    Area of Science:

    • Cell Biology
    • Virology
    • Biochemistry

    Background:

    • Adenovirus type 2 (Ad2) is a common human pathogen.
    • The interaction between viruses and host cells can lead to various cellular responses.
    • Understanding viral effects on cellular metabolism is crucial for virology research.

    Purpose of the Study:

    • To investigate the effect of Ad2 on the release of specific molecules from KB cells.
    • To characterize the Ad2-dependent release of choline and its metabolites.
    • To determine the conditions influencing Ad2-mediated choline release.

    Main Methods:

    • KB cells were labeled with radioactive isotopes (51Cr, [35S]methionine, [3H]choline, etc.).
    • Cells were treated with Ad2 under varying conditions (concentration, pH, time).

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  • Released molecules were quantified, and choline metabolites were identified using biochemical assays.

    • Main Results:

    • Ad2 treatment caused significant release of [3H]choline compared to 51Cr or [35S]methionine.
    • Choline release was dependent on Ad2 concentration, incubation time, and medium pH (optimal at 6.0-6.5).
    • Over 95% of released choline was identified as choline phosphate; heat-inactivated Ad2 and fetal calf serum abolished this release.

    Conclusions:

    • Ad2 actively induces the release of choline from KB cells, primarily in the form of choline phosphate.
    • The Ad2-mediated release mechanism is sensitive to temperature and serum factors.
    • This Ad2-induced release of choline phosphate may represent a novel viral-host interaction.