Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Energy to Drive Translocation01:37

Energy to Drive Translocation

2.0K
Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...
2.0K
The Movement of Organelles and Vesicles01:43

The Movement of Organelles and Vesicles

4.3K
In eukaryotic cells,  cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
4.3K
Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

3.1K
Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
3.1K
Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

2.5K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
2.5K
Protein Transport into the Inner Mitochondrial Membrane01:34

Protein Transport into the Inner Mitochondrial Membrane

3.6K
Nuclear encoded mitochondrial precursors are imported to the inner membrane in a multistep process involving two separate translocons, TIM22 and TIM23. TIM23 is a cation-selective pore that remains closed by the N terminal segment of the protein. Negative charges on the TIM23 act as a receptor for the incoming precursor, pulling the positively charged matrix-targeting sequence for peptide insertion and translocation.
Transport of mitochondrial precursors across the TIM23 channel is driven by...
3.6K
Rab Cascades01:25

Rab Cascades

2.6K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The impact of supramolecular self-association of organocatalysts on catalytic performance.

Nature reviews. Chemistry·2025
Same author

3,3'-Linked BINOL macrocycles: optimized synthesis of crown ethers featuring one or two BINOL units.

Beilstein journal of organic chemistry·2025
Same author

A CoA-Transferase and Acyl-CoA Dehydrogenase Convert 2-(Carboxymethyl)cyclohexane-1-Carboxyl-CoA During Anaerobic Naphthalene Degradation.

Environmental microbiology·2024
Same author

Steric Engineering of Rotaxane Catalysts: Benefits and Limits of Using the Mechanical Bond in Catalyst Design.

Chemistry (Weinheim an der Bergstrasse, Germany)·2024
Same author

Molecular folding governs switchable singlet oxygen photoproduction in porphyrin-decorated bistable rotaxanes.

Communications chemistry·2024
Same author

Stepwise Dissipative Control of Multimodal Motion in a Silver(I) Catenate.

Angewandte Chemie (International ed. in English)·2024

Related Experiment Video

Updated: May 15, 2025

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis
11:09

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

Published on: October 30, 2014

9.3K

Directional Macrocycle Transport, Release, and Recapture Enabled by a Rotaxane Transporter.

Sohom Kundu1,2, Shubhadip Mallick1, Jan Riebe1

  • 1Faculty of Chemistry (Organic Chemistry) and Center for Nanointegration Duisburg-Essen (CENIDE), University of Duisburg-Essen, Universitätsstrasse 7, 45141, Essen, Germany.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|April 7, 2025
PubMed
Summary

This study presents a novel rotaxane-based molecular transporter capable of directional macrocycle movement, release, and recapture. This iterative system demonstrates a new design for molecular machines and supramolecular chemistry applications.

Keywords:
molecular devicesmolecular release systemsmolecular transportersrotaxanessupramolecular chemistry

More Related Videos

Cargo Loading onto Kinesin Powered Molecular Shuttles
09:00

Cargo Loading onto Kinesin Powered Molecular Shuttles

Published on: November 3, 2010

10.4K
Measuring Axonal Cargo Transport in Mouse Primary Cortical Cultured Neurons
04:39

Measuring Axonal Cargo Transport in Mouse Primary Cortical Cultured Neurons

Published on: February 24, 2023

281

Related Experiment Videos

Last Updated: May 15, 2025

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis
11:09

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

Published on: October 30, 2014

9.3K
Cargo Loading onto Kinesin Powered Molecular Shuttles
09:00

Cargo Loading onto Kinesin Powered Molecular Shuttles

Published on: November 3, 2010

10.4K
Measuring Axonal Cargo Transport in Mouse Primary Cortical Cultured Neurons
04:39

Measuring Axonal Cargo Transport in Mouse Primary Cortical Cultured Neurons

Published on: February 24, 2023

281

Area of Science:

  • Supramolecular Chemistry
  • Materials Science
  • Chemical Engineering

Background:

  • Molecular machines require precise control over component movement and function.
  • Rotaxanes offer a unique platform for constructing mechanically interlocked molecules with tunable properties.

Purpose of the Study:

  • To construct and demonstrate a rotaxane-based transporter for directional macrocycle transport, release, and recapture.
  • To investigate the iterative operation of such a molecular transporter.

Main Methods:

  • Synthesis of a rotaxane featuring a dibenzo-24-crown-8 macrocycle and specific stations (dibenzylammonium/methyl triazolium) and stoppers (anthracene/triisopropylsilyl-acetylene).
  • Utilized chemical inputs (base, fluoride) to induce directional shuttling, release, and reassembly of the rotaxane.
  • Employed CuAAC click chemistry to reestablish the mechanical bond for iterative cycling.

Main Results:

  • Achieved directional shuttling of the macrocycle between stations in response to chemical stimuli.
  • Demonstrated the release of the macrocycle and half-thread upon stopper cleavage by fluoride.
  • Successfully recaptured the macrocycle and reassembled the rotaxane transporter for iterative operation.

Conclusions:

  • The developed rotaxane transporter enables controlled, directional macrocycle transport, release, and recapture.
  • The system exhibits iterative functionality, paving the way for reusable molecular machines.
  • This molecular design offers a promising approach for advanced supramolecular systems and nanodevices.