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Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
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1,3,5-Tricaffeoylquinic Acid from Ipomoea batatas Vines Induced Ovarian Cancer Cell Apoptosis and Inhibited

Dahae Lee1, Jaekyoung Kim2, Soyoon Baek3

  • 1College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea.

Biomolecules & Therapeutics
|April 8, 2025
PubMed

Insights

1,3,5-tricaffeoylquinic acid from Ipomoea batatas vines induces apoptosis in ovarian cancer cells and inhibits angiogenesis, showing potential for ovarian cancer treatment.

Area of Science:

  • Phytochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • Ovarian cancer metastasis involves direct invasion and angiogenesis.
  • Targeting apoptosis and angiogenesis are key anticancer strategies.
  • Ipomoea species show promise in ovarian cancer treatment.

Purpose of the Study:

  • To investigate the apoptosis-inducing and anti-angiogenic effects of compounds from Ipomoea batatas vines (IBV).
  • To identify potent compounds from IBV for ovarian cancer therapy.

Main Methods:

  • Phytochemical isolation and verification of eight compounds from IBV.
  • Cytotoxicity assays on A2780 human ovarian cancer cells.
  • Western blot analysis for apoptosis and angiogenesis markers.
  • Inhibition of tubule formation assays in human umbilical vein endothelial cells (HUVECs).
  • LC-MS/MS method development for compound quantification.

Main Results:

  • 1,3,5-tricaffeoylquinic acid (compound 5) exhibited the highest cytotoxicity, inducing >37% apoptosis and reducing viability to <25% in A2780 cells at 100 μM.
  • Compound 5 modulated apoptosis markers: increased cleaved caspase-8, Bax, cleaved PARP, caspase-3/9, and decreased cleaved Bcl-2.
  • Compound 5 inhibited HUVEC tubule formation and suppressed key angiogenesis signaling proteins (VEGFR2, ERK, PI3K, Akt, mTOR).

Conclusions:

  • 1,3,5-tricaffeoylquinic acid demonstrates significant potential for ovarian cancer treatment.
  • Its efficacy stems from inducing apoptosis in cancer cells and inhibiting angiogenesis.
Keywords:
1,3,5-tricaffeoylquinic acidApoptosisLC-MS/MSOvarian cancer

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