Genetic engineering for SIT application: a fruit fly-focused review

Sterile insect technique (SIT) has become a key component of efficient pest control. Fruit fly pests from the Drosophilidae and Tephritidae families pose a substantial and overwhelmingly increasing threat to the agricultural industry, aggravated by climate change and globalization among other contributors. In this review, we discuss the advances in genetic engineering aimed to improve the SIT-mediated fruit fly pest control. This includes SIT enhancement strategies such as novel genetic sexing strain and female lethality approaches. Self-pervasive X-shredding and X-poisoning sex distorters, alongside gene drive varieties are also reviewed. The self-limiting precision-guided SIT, which aims to tackle female removal and male fertility via CRISPR/Cas9, is additionally introduced. By using examples of existing genetic tools in the fruit fly pests of interest, as well as model species, we illustrate that the population control intensity may be modulated depending on strategy selection.