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Shade-Induced Regulation of Cell Wall Thickening in Moso Bamboo: A Molecular Pathway Involving XTH Activity
Yali Xie1, Junlei Xu1, Xiangyi Li1
1Key Laboratory of National Forestry and Grassland Administration on Bamboo & Rattan Science and Technology, International Center for Bamboo and Rattan, State Forestry and Grassland Administration, Beijing, China.
Abstract:
How plants adapt to shade and perform specific morphogenesis is one of the secrets of the kingdom, and unravelling the underlying molecular mechanisms is crucial. Scanning electron microscope results have revealed that a low R/FR ratio (indicating shade conditions) inhibited the cell wall thickness of parenchyma cells and sieve tube cells of Moso bamboo (Phyllostachys edulis). Xyloglucan Endotransglucosylase/hydrolase3 (PheXTH3) was identified as a circadian rhythm gene responsive to a low R/FR ratio by qRT-PCR analysis, and it showed peak activity in the vascular system. PheXTH3 enhanced interfascicular fibre cell wall thickening and lignin accumulation in stable transgenic Arabidopsis thaliana lines. A low R/FR ratio downregulated PheXTH3 expression, subsequently suppressing cell wall thickening in vessel and interfascicular fibre cells. Both Yeast One-Hybrid experiments and Dual-LUC assays revealed that WOX3b1, AP2-39, and XTH3 may form a regulatory pathway. Collectively, we proposed a WOX3b1-AP2-39-XTH3 molecular pathway mediated by the ratio of low R/FR, which may regulate the thickening of cell wall in Moso bamboo.
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