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Related Concept Videos

CRISPR01:59

CRISPR

48.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
48.6K
CRISPR and crRNAs02:53

CRISPR and crRNAs

16.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.3K

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Related Experiment Video

Updated: May 15, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

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Progress and challenges in CRISPR/Cas applications in microalgae.

Quynh-Giao Tran1, Trang Thi Le1,2, Dong-Yun Choi1

  • 1Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.

Journal of Microbiology (Seoul, Korea)
|April 8, 2025
PubMed
Summary
This summary is machine-generated.

CRISPR gene editing advances microalgal biotechnology by overcoming challenges in genetic modification. These tools enhance precision and efficiency for industrial applications.

Keywords:
CRISPR/Casgenome editingmicroalgaeoff-target effectsynthetic biologytransformation efficiency

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • Microalgal production has advanced significantly since the 1950s, moving towards metabolic engineering for industrial needs.
  • Effective genetic modification in microalgae faces challenges like low transformation efficiency and interspecies genetic variation.

Purpose of the Study:

  • To review recent advancements in Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems applied to microalgae.
  • To focus on improving gene editing precision and efficiency while addressing organism-specific challenges in microalgae.

Main Methods:

  • Review of recent literature on CRISPR applications in microalgae.
  • Focus on Class 2 CRISPR-associated (Cas) proteins like Cas9 and Cas12a.
  • Discussion of emerging CRISPR-based strategies for microalgal cellular barriers.

Main Results:

  • CRISPR technologies offer powerful tools for precise genome editing in microalgae.
  • Advancements are improving gene editing precision and efficiency in diverse microalgal species.
  • Class 2 CRISPR systems (Cas9, Cas12a) show notable success.

Conclusions:

  • CRISPR/Cas strategies hold significant promise for advancing microalgal biotechnology.
  • Overcoming organism-specific challenges is key to unlocking CRISPR's full potential in microalgae.
  • Future perspectives involve further tailoring CRISPR/Cas systems for microalgal applications.