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Updated: Jun 17, 2026

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Published on: May 10, 2022
De novo design of inducibly assembling multi-component filaments
Hao Shen1,2, Eric M Lynch1, Noel Jameson3
1Department of Biochemistry, University of Washington, Seattle, WA, USA.
None:
The design of multi-component nanomaterials is an outstanding challenge. Here, we describe the computational design of protein filaments with two or three distinct structural components that assemble into micron-scale, well-ordered fibers when mixed. CryoEM structure determination of four fiber designs was close to the computational design models. Filament assembly can be initiated by mixing the components, and modulated by addition and/or phosphorylation of designed regulatory subunits. This work demonstrates that regulatable multi-component protein filament systems can now be designed, opening the door to a wide range of engineered materials.
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