Exploring Protein Expression Profiles in Lung Cancer Insufficient Microwave Ablation: Implications for Recurrence
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Updated: May 15, 2025
Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
Published on: April 29, 2014
Peng Yan1, Ruimei Yuan2, Zheng Li3
1Department of Oncology, Jinan Central Hospital, Shandong First Medical University, Jinan, People's Republic of China.
Background:
Insufficient ablation is a significant factor contributing to the recurrence of non-small cell lung cancer (NSCLC), and it is of great significance to explore the protein expression profile of lung cancer cells after insufficient ablation.
Methods:
We establish an insufficient microwave ablation model of lung cancer xenograft in mice, identify differentially expressed proteins (DEPs) and involved signaling pathways through proteomic sequencing, and confirm proteins expression via immunohistochemistry (IHC). Utilizing The Cancer Genome Atlas (TCGA) dataset, we investigate proteins associated with human lung cancer prognosis.
Results:
Proteomic sequencing results reveal that 99 proteins exhibited differential expression levels. Subsequent Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicate that the DEPs are significantly enriched in metabolic processes. Several DEPs are identified and subsequently confirmed through immunohistochemistry (IHC). Among these proteins, CTP synthase 1 (CTPS1) and Thymidylate synthetase (TYMS), both of which play roles in nucleotide metabolism, are found to be significantly associated with the survival outcomes of patients with lung cancer.
Conclusion:
Insufficient ablation can cause alterations in nucleotide metabolism, potentially leading to recurrence and metastasis.
Insights
Insufficient ablation in non-small cell lung cancer (NSCLC) alters nucleotide metabolism. This study identifies key proteins like CTPS1 and TYMS linked to patient survival, offering insights into cancer recurrence.
Area of Science:
- Oncology
- Molecular Biology
- Proteomics
Background:
- Insufficient ablation is a major cause of non-small cell lung cancer (NSCLC) recurrence.
- Understanding protein expression changes post-ablation is crucial for improving treatment outcomes.
Purpose of the Study:
- To investigate the protein expression profile of lung cancer cells following insufficient ablation.
- To identify proteins and pathways associated with NSCLC recurrence and patient prognosis.
Main Methods:
- Established an insufficient microwave ablation model in mice.
- Utilized proteomic sequencing to identify differentially expressed proteins (DEPs) and signaling pathways.
- Confirmed protein expression via immunohistochemistry (IHC) and analyzed TCGA data for prognostic associations.
Main Results:
- Identified 99 differentially expressed proteins (DEPs) after insufficient ablation.
- Found DEPs significantly enriched in metabolic processes, particularly nucleotide metabolism.
- CTP synthase 1 (CTPS1) and Thymidylate synthetase (TYMS) were associated with lung cancer patient survival.
Conclusions:
- Insufficient ablation induces alterations in nucleotide metabolism.
- These metabolic changes may contribute to NSCLC recurrence and metastasis.
- CTPS1 and TYMS are potential prognostic markers for NSCLC.
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