Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phosphodiester Linkages

Phosphodiester Linkages

Overview
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...

Aryldiazonium Salts to Azo Dyes: Diazo Coupling

Aryldiazonium Salts to Azo Dyes: Diazo Coupling

The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development and evaluation of a deep learning-assisted diagnostic support system for radiographer preliminary clinical evaluation of intracranial hemorrhage.

PeerJ·2026

Same author

Managing Oral RAE Tube Malpositioning After Neck Extension.

Anesthesia progress·2026

Same author

Charge-Balanced Design for Redox-Responsive Disassembly of Ampholytic β-Sheet Peptide Nanofibers.

Polymers·2026

Same author

<i>Wedelia trilobata</i> (L.) Leaf Extract Induces Autophagy-Mediated Cell Death in HT-29 Colorectal Cancer Cells via Suppression of the Akt/mTOR Signaling Axis.

International journal of molecular sciences·2026

Same author

Effects of "antifreeze" oligopeptides and electric fields on unidirectional ice growth in thin water layers.

Soft matter·2026

Same author

Optochemical elucidation of a critical role of the incomplete spindle assembly checkpoint in zebrafish development.

Communications biology·2026

Search research articles

Related Experiment Video

Updated: May 15, 2025

Single Molecule Analysis of Laser Localized Psoralen Adducts

Single Molecule Analysis of Laser Localized Psoralen Adducts

Published on: April 20, 2017

Point Mutation Selective Photo-Cross-Linking Reactions by Diazirine-Derivatized ODNs.

Kentaro Kobata¹, Daiki Unita¹, Kazuya Matsuo¹

¹Faculty of Molecular Chemistry and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.

The Journal of Organic Chemistry

|April 8, 2025

Summary

Researchers developed novel photo-cross-linking oligonucleotides (U-ODN) to target and inhibit mutated KRAS messenger RNAs (mRNAs). This new method shows promise for selectively targeting point mutations in genes like KRAS.

More Related Videos

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Published on: August 19, 2012

Related Experiment Videos

Last Updated: May 15, 2025

Single Molecule Analysis of Laser Localized Psoralen Adducts

Single Molecule Analysis of Laser Localized Psoralen Adducts

Published on: April 20, 2017

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Published on: August 19, 2012

Area of Science:

Molecular Biology
Oligonucleotide Chemistry

Background:

KRAS gene mutations, primarily point mutations, are common in various cancers.
Targeting these specific mutations for therapeutic intervention remains a significant challenge in molecular biology.

Purpose of the Study:

To develop novel photo-cross-linking oligonucleotides (U-ODN) capable of selectively targeting and inhibiting KRAS point mutations.
To investigate the reactivity and specificity of these U-ODN against wild-type and mutated RNA sequences.

Main Methods:

Synthesis of novel photo-cross-linking oligonucleotides (U-ODN) incorporating a diazirine group on uridine derivatives.
Photo-cross-linking assays were performed using both wild-type and mutated RNA targets.

Main Results:

U-ODN demonstrated efficient and selective photo-cross-linking with mutated RNAs.
The reactivity was specific to mutated RNAs with cytidine, guanosine, or uridine at the position adjacent to the U-ODN binding site.

Conclusions:

U-ODN represents a promising tool for selectively inhibiting the activity of mRNAs containing point mutations.
This approach offers potential for developing targeted therapies against diseases driven by KRAS mutations.