Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Serum Laboratory Studies, Stool Test, Breath Test

Serum Laboratory Studies, Stool Test, Breath Test

Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Actin cytoskeletal dynamics in hepatic myofibroblasts and fibrosis.

Nature reviews. Gastroenterology & hepatology·2026

Same author

The impact of COVID-19 on alcohol-associated hepatitis and alcohol-associated cirrhosis.

The American journal of the medical sciences·2026

Same author

Caregiver Involvement and Burden and Patient Quality of Life in Patients With Decompensated Cirrhosis.

The American journal of gastroenterology·2026

Same author

Response to Letters to the Editor Regarding 'Terlipressin for Hepatorenal Syndrome in Patients With Early-Stage Acute-On-Chronic Liver Failure'.

Liver international : official journal of the International Association for the Study of the Liver·2026

Same author

Lactulose-induced Hypernatremia in Cirrhotic Patients With Hepatic Encephalopathy.

Journal of clinical gastroenterology·2026

Same author

Research Communication: The Risk and Outcomes of Gastrointestinal Bleeding in Patients Using Warfarin or Direct Oral Anticoagulants.

Alimentary pharmacology & therapeutics·2025

Search research articles

Related Experiment Video

Updated: May 16, 2025

Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

Published on: June 8, 2020

The Utility of Multitarget Stool DNA Testing in Community-Based Clinical Practice.

Abhinav K Rao^1,2, Shivam Kalra¹, Danny Tran¹

¹Division of Internal Medicine, Trident Medical Center, North Charleston, South Carolina, USA.

The American Journal of Gastroenterology

|April 8, 2025

Summary

Multitarget stool DNA (MT-sDNA) tests detected colorectal cancer (CRC) at a lower rate than expected. Most positive MT-sDNA results in this primary care screening cohort were false positives, questioning test effectiveness for average-risk individuals.

Keywords:

Cologuard colon cancer colon cancer screening colonoscopy fecal immunochemical test multitarget stool DNA tests

More Related Videos

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Related Experiment Videos

Last Updated: May 16, 2025

Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

Published on: June 8, 2020

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Area of Science:

Oncology
Gastroenterology
Preventive Medicine

Background:

Multitarget stool DNA (MT-sDNA) tests, such as Cologuard, are recommended for colorectal cancer (CRC) screening.
The US Preventive Services Task Force suggests screening every 1-3 years.
This study evaluated MT-sDNA test performance and subsequent colonoscopy findings in a primary care setting.

Purpose of the Study:

To assess the diagnostic performance of MT-sDNA testing for colorectal cancer (CRC).
To analyze colonoscopy findings following a positive MT-sDNA test result.
To evaluate the effectiveness of MT-sDNA screening in an average-risk population.

Main Methods:

Retrospective cohort study using electronic health records from 35 primary care facilities (Winter 2019-Spring 2023).
Included patients who underwent MT-sDNA testing, excluding high-risk individuals or those with prior negative tests/colonoscopies.
Pathology assessment included CRC and advanced adenomas.

Main Results:

Of 3,119 patients who completed MT-sDNA tests, 482 (15%) were positive.
Follow-up colonoscopies in 253 patients revealed 10 (4%) with CRC and 61 (24%) with advanced adenomas.
A significant majority (73%) of positive MT-sDNA tests did not lead to a diagnosis of CRC or advanced adenomas.

Conclusions:

The observed CRC detection rate (0.2%-0.3%) was lower than anticipated for a screening cohort.
A high false-positive rate (73%) for advanced adenomas or CRC was observed among patients with positive MT-sDNA tests.
Findings suggest a need to re-evaluate the effectiveness of MT-sDNA screening in average-risk populations.