Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Structure and Function of Platelets01:18

Structure and Function of Platelets

906
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
906
Production of Formed Elements01:34

Production of Formed Elements

1.3K
Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...
1.3K
Hematopoiesis01:21

Hematopoiesis

4.9K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
4.9K
Overview of Hematopoiesis01:20

Overview of Hematopoiesis

3.6K
Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
3.6K
Lineage Commitment01:21

Lineage Commitment

2.9K
Commitment is the  process whereby stem cells:
2.9K
Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

1.2K
Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: The AIM2 inflammasome exacerbates atherosclerosis in clonal haematopoiesis.

Nature·2026
Same author

Engraftment of wild-type alveolar type II epithelial cells in surfactant protein C deficient mice.

NPJ Regenerative medicine·2026
Same author

Advances in our understanding of distal progenitors in idiopathic pulmonary fibrosis: implications for novel therapeutics.

The European respiratory journal·2025
Same author

Mitofusin agonists enhances long-term engraftment and potency of HSC cultures <i>in vivo</i>.

bioRxiv : the preprint server for biology·2025
Same author

Rapid and efficient protocol for optical clearing of mouse intestinal tissues for enhanced fluorescence imaging and 3D reconstruction.

STAR protocols·2025
Same author

Theranostic methodology for ex vivo donor lung rehabilitation.

Med (New York, N.Y.)·2025
Same journal

Dynamic myeloid suppressor states in cancer and inflammation and their therapeutic potential.

Current opinion in hematology·2026
Same journal

Factor XIa inhibition for the prevention of thrombosis: mechanism, clinical trial signals, and indication-specific positioning.

Current opinion in hematology·2026
Same journal

Nutrition as a regulator of hematopoietic stem cell biology and transplantation.

Current opinion in hematology·2026
Same journal

From biomimicry to clinical actionability: rethinking high-shear thrombosis as a mechanobiological system.

Current opinion in hematology·2026
Same journal

Bidirectional relationship between metabolic and thrombotic disease mechanisms.

Current opinion in hematology·2026
Same journal

The dual role of the brain-derived neurotrophic factor as a regulator of hemostasis and thrombotic risk.

Current opinion in hematology·2026
See all related articles

Related Experiment Video

Updated: May 15, 2025

Isolation of Mouse Megakaryocyte Progenitors
10:30

Isolation of Mouse Megakaryocyte Progenitors

Published on: May 20, 2021

6.3K

Direct megakaryopoiesis.

Hans-Willem Snoeck1,2,3

  • 1Columbia Center for Stem Cell Therapies/Columbia Center for Human Development, Department of Medicine.

Current Opinion in Hematology
|April 8, 2025
PubMed
Summary
This summary is machine-generated.

A direct pathway for megakaryocyte production from hematopoietic stem cells (HSCs) bypasses traditional progenitors. This newly characterized route generates distinct megakaryocyte progenitors and may influence thrombotic risk in aging and myeloproliferative neoplasms.

Keywords:
agingemergency hematopoiesishematopoietic stem cellsmegakaryocytesplatelets

More Related Videos

In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy
08:15

In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy

Published on: September 8, 2021

2.7K
Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
09:46

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Published on: December 27, 2017

19.5K

Related Experiment Videos

Last Updated: May 15, 2025

Isolation of Mouse Megakaryocyte Progenitors
10:30

Isolation of Mouse Megakaryocyte Progenitors

Published on: May 20, 2021

6.3K
In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy
08:15

In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy

Published on: September 8, 2021

2.7K
Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
09:46

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Published on: December 27, 2017

19.5K

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Megakaryocytes, essential for platelet production, traditionally arise from stepwise differentiation of hematopoietic stem cells (HSCs).
  • Emerging evidence suggested a more direct differentiation pathway, but its characteristics and significance remained unclear.

Purpose of the Study:

  • To investigate the existence and characteristics of a direct megakaryocyte differentiation pathway from HSCs.
  • To understand the functional and phenotypic implications of this direct pathway compared to the classical route.

Main Methods:

  • Utilized fate-mapping and single-cell transplantation techniques.
  • Analyzed molecular characteristics and phenotypic differences of megakaryocytes derived from distinct pathways.

Main Results:

  • Unequivocally demonstrated a direct megakaryocyte differentiation pathway originating from a specific subset of 'top' HSCs.
  • This pathway bypasses multipotential progenitors and is enhanced by hematopoietic stress, inflammation, and aging.
  • Generated phenotypically distinct megakaryocyte progenitors, yielding more reactive platelets, and may be active in myeloproliferative neoplasms.

Conclusions:

  • Provides novel insights into HSC biology and hematopoiesis.
  • Enhances understanding of hematological recovery post-myeloablation and the aging hematopoietic system.
  • Suggests the direct pathway's contribution to age-related thrombotic events and myeloproliferative neoplasms.