Related Experiment Video
Updated: May 15, 2025
Murine Fecal Isolation and Microbiota Transplantation
Published on: May 26, 2023
A microbiota pattern associated with cardiovascular events in secondary prevention: the CORDIOPREV study
Javier Arenas-Montes1,2,3,4, Juan F Alcala-Diaz1,2,3,4, Helena Garcia-Fernandez1,2,3,4
1Lipids and Atherosclerosis Unit, Department of Internal Medicine, Hospital Universitario Reina Sofía, Cordoba 14004, Spain.
Insights
Gut microbiota profiles predict major adverse cardiovascular events (MACE) in coronary heart disease (CHD) patients. A novel risk score based on intestinal bacteria and endotoxemia may improve secondary prevention strategies for cardiovascular disease (CVD).
Area of Science:
- Microbiome research
- Cardiovascular disease research
- Preventive cardiology
Background:
- Secondary prevention of cardiovascular disease (CVD) remains challenging.
- Intestinal microbiota composition may identify patients at risk for new cardiovascular events.
- Understanding gut microbiota differences can refine CVD risk prediction.
Purpose of the Study:
- To compare baseline gut microbiota in coronary heart disease (CHD) patients who experienced major adverse cardiovascular events (MACE) versus those who did not.
- To develop a risk score for MACE based on gut microbial profiles.
- To investigate the role of lipopolysaccharide (LPS) in MACE prediction.
Main Methods:
- Analysis of fecal samples from 679 CHD patients within the CORDIOPREV study using 16S metagenomics.
- Application of Quiime2 software for microbial analysis and random survival forest for predictive modeling.
- Measurement of lipopolysaccharide (LPS) levels using the limulus amoebocyte lysate test.
Main Results:
- Ten bacterial taxa were identified as having significant predictive power for MACE.
- A developed intestinal microbiota risk score showed a hazard ratio of 2.01 for MACE incidence.
- Patients experiencing MACE exhibited a greater post-prandial increase in LPS levels (P = .005).
Conclusions:
- Gut microbiota composition is associated with MACE risk in CHD patients.
- The findings support a link between intestinal microbiota, endotoxemia, and cardiovascular events.
- A microbiota-based risk score could aid in identifying high-risk individuals for improved CVD secondary prevention.
Background And Aims:
Preventing new cardiovascular events in patients with established cardiovascular disease (CVD) is a daunting task for clinicians. Intestinal microbiota may help identify patients at risk, thus improving the strategies of secondary prevention. The aim of this study was to evaluate the baseline differences between the gut microbiota from coronary heart disease (CHD) patients suffering new major adverse cardiovascular events (MACEs) in the following 7 years, compared with CHD patients who did not undergo new MACE in this period, and to build a score associated with the risk of suffering new MACE.
Methods:
Within the framework of the CORDIOPREV study, a clinical trial that involved 1002 patients with CHD, intestinal microbiota was examined in patients with available faecal samples (n = 679, 132 MACE), through 16S metagenomics on the Illumina MiSeq and Quiime2 software. Lipopolysaccharide (LPS) was measured using limulus amoebocyte lysate test.
Results:
Random survival forest identified 10 bacterial taxa with a higher predictive power for MACE incidence. Receiver operating characteristic curves yielded an area under the curve of 65.2% (59.1%-71.3%) in the training set and 68.6% (59.3%-77.9%) in the validation set. The intestinal microbiota risk score was associated with a MACE incidence hazard ratio of 2.01 (95% confidence interval 1.37-3.22). Lipopolysaccharide analysis showed a greater LPS post-prandial fold change in the MACE group (P = .005).
Conclusions:
These results reinforce the relationship between intestinal microbiota and CVD and suggest that a microbiota profile is associated with MACE in CHD patients, in addition to higher endotoxaemia.
More Related Videos
Related Concept Videos
01:27Psychoneuroimmunology: Cardiovascular Disease
A key area of focus in PNI is the relationship between stress and coronary...
01:19Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
01:23Assessment of the Cardiovascular System I: Subjective Data
Initial Enquiry
Ask the patient about their primary concern and thoroughly explore all reported symptoms.
Medical History
Investigate past illnesses affecting the cardiovascular system, such as angina, anemia, rheumatic fever, congenital heart disease, stroke, thrombophlebitis, dysrhythmias, varicosities
Inquire about symptoms...