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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Updated: May 12, 2026

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

Published on: October 16, 2015

Surface-Defect-Involved Chemiluminescence Boosted by Gold-Silver Bimetallic Nanoclusters for Bioanalysis.

Tongtong Zhai^1,2, Luyao Zhang^1,2, Sipeng Tian^1,2

¹College of Chemistry, Jilin University, Changchun, Jilin 130012, China.

Analytical Chemistry

|April 8, 2025

Summary

This summary is machine-generated.

Researchers developed glutathione-coated gold-silver bimetallic nanoclusters (GSH-AuAg NCs) that significantly boost chemiluminescence (CL) emission. This novel approach enhances analytical sensitivity for detecting analytes like hydrogen peroxide and glucose.

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Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

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Published on: March 22, 2020

Area of Science:

Analytical Chemistry
Materials Science
Nanotechnology

Background:

Traditional chemiluminescence (CL) luminophores have limited emission efficiency, hindering analytical applications.
Metal nanoclusters (NCs) show promise for luminescence but their CL performance is often overlooked.
Developing novel probes with enhanced CL emission is crucial for improving analytical sensitivity.

Purpose of the Study:

To explore the chemiluminescence properties of bimetallic nanoclusters (NCs) as novel CL emitters.
To optimize glutathione-coated gold-silver bimetallic NCs (GSH-AuAg NCs) for enhanced CL emission.
To investigate the mechanism behind the boosted CL and demonstrate their application in sensing.

Main Methods:

Synthesis and optimization of glutathione-coated Au-Ag bimetallic NCs (GSH-AuAg NCs) by varying precursor ratios.
Characterization of CL properties using potassium permanganate (KMnO4) as an oxidant.
Analysis of CL spectra and comparison with photoluminescence (PL) spectra to elucidate the emission mechanism.
Construction of a sensing platform for H2O2 and glucose detection.

Main Results:

Optimized GSH-AuAg NCs achieved maximum CL response at a Au:Ag molar ratio of 5:1.
Bimetallic GSH-AuAg NCs exhibited a 6.2-fold increase in CL emission compared to monometallic NCs due to synergistic effects.
Surface-defect-involved CL emission was observed around 750 nm, with a red shift compared to PL.
A sensing platform demonstrated the potential for detecting H2O2 and glucose using the GSH-AuAg NCs/oxidant system.

Conclusions:

Synergistic effects in bimetallic GSH-AuAg NCs significantly enhance chemiluminescence emission efficiency.
The developed GSH-AuAg NCs show great potential as highly efficient CL emitters for analytical applications.
This work opens new avenues for utilizing bimetallic nanoclusters in sensitive detection systems.