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Regulation of Expression at Multiple Steps01:23

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Related Experiment Video

Updated: May 15, 2025

Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae
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Transcriptomic analysis reveals the function of m6A regulators in aged cochlea.

Yanbing Lai1, Bo Su1, Xiaodi Wang1

  • 1Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Department of Otorhinolaryngology Head and Neck Surgery, Wuhan, China.

Brazilian Journal of Otorhinolaryngology
|April 8, 2025
PubMed
Summary
This summary is machine-generated.

This study investigated N6-methyladenosine (m6A) regulators in aging mouse cochleae, identifying ALKBH5 and YTHDC1 as upregulated in older mice. These regulators show potential as diagnostic markers for age-related hearing loss (presbycusis).

Keywords:
Aged cochleaImmune-related genesMiceTranscriptomicsm6A regulators

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Area of Science:

  • Molecular Biology
  • Genetics
  • Otolaryngology

Background:

  • Presbycusis, or age-related hearing loss, is a prevalent health issue with incompletely understood mechanisms.
  • N6-methyladenosine (m6A) modification plays a role in gene expression and cellular functions, implicated in various diseases.
  • The specific role of m6A regulators in the development of presbycusis remains largely unexplored.

Purpose of the Study:

  • To determine the expression patterns of m6A regulators in the cochleae of young versus old mice.
  • To investigate the potential involvement of m6A regulators in the aging process associated with presbycusis.

Main Methods:

  • Transcriptome sequencing of cochlear tissues from young (2-month-old) and old (24-month-old) mice.
  • Bioinformatic analysis including differential gene expression and functional enrichment analysis.
  • Construction of protein-protein interaction and transcription factor-miRNA networks to elucidate regulatory mechanisms.

Main Results:

  • ALKBH5 and YTHDC1 were significantly upregulated in the cochleae of aged mice.
  • These upregulated regulators correlated with immune-related pathways, molecular subtypes, and immune cell infiltration in aged cochleae.
  • Receiver Operating Characteristic (ROC) curve analysis indicated a high diagnostic potential for ALKBH5 and YTHDC1 in presbycusis.

Conclusions:

  • This study provides a molecular basis for understanding the role of m6A regulators in presbycusis.
  • ALKBH5 and YTHDC1 are identified as novel potential molecular markers for age-related hearing impairment.
  • The findings suggest a link between m6A modification, immune responses, and the pathogenesis of presbycusis.