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Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

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The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
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Related Experiment Video

Updated: May 15, 2025

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
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Sanguinarine Inhibits Cell Growth in EBV-Positive Diffuse Large B-Cell Lymphoma.

Suli Lu1, Dae-Jung Yang2

  • 1Department of Medicine, Hunan Polytechnic of Environment and Biology, Hengyang, Hunan 421005, China.

Archivum Immunologiae Et Therapiae Experimentalis
|April 8, 2025
PubMed
Summary

Sanguinarine (SAG) inhibits the growth of Epstein-Barr virus-positive diffuse large B-cell lymphoma (DLBCL) by inducing cell cycle arrest and apoptosis. This compound targets the Wnt/β-catenin pathway, offering potential therapeutic strategies for DLBCL.

Keywords:
ApoptosisCell cycleEBV-positive DLBCLSanguinarineWnt/β-catenin

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Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Diffuse large B-cell lymphoma (DLBCL) is an aggressive non-Hodgkin lymphoma.
  • Epstein-Barr virus (EBV) positivity is a feature in a subset of DLBCL cases.
  • Targeting specific molecular pathways is crucial for developing novel DLBCL therapies.

Purpose of the Study:

  • To investigate the anti-cancer effects of Sanguinarine (SAG) on EBV-positive DLBCL cell lines.
  • To elucidate the underlying molecular mechanisms of SAG's action.
  • To explore SAG as a potential therapeutic agent for DLBCL.

Main Methods:

  • Utilized EBV-positive DLBCL cell lines (FARAGE, GM12878S).
  • Assessed cell proliferation using Cell Counting Kit-8 and bromodeoxyuridine assays.
  • Analyzed cell cycle arrest and apoptosis via flow cytometry and immunoblotting.
  • Investigated molecular mechanisms, including the Wnt/β-catenin pathway, using immunoblotting.

Main Results:

  • SAG significantly suppressed the proliferation of EBV-positive DLBCL cells.
  • SAG treatment led to cell cycle arrest and induced apoptosis in these cells.
  • Mechanistically, SAG was found to suppress the Wnt/β-catenin signaling pathway.

Conclusions:

  • Sanguinarine exhibits potent anti-proliferative and pro-apoptotic effects on EBV-positive DLBCL cells in vitro.
  • The Wnt/β-catenin pathway is a key target of SAG in suppressing DLBCL progression.
  • SAG demonstrates potential as a therapeutic candidate for EBV-positive DLBCL.