4D-FastDIA proteomic analysis of pedal mucus in Pacific abalone Haliotis discus hannai heat-resistance group

This study aimed to investigate the immune mechanisms of pedal mucus in Pacific abalone Haliotis discus hannai from different populations. Proteomic differences between the pedal mucus of the control group and the heat-resistance group of H. discus hannai were comparatively analyzed, and the proteins were annotated and analyzed to understand the related functions and roles of differentially expressed proteins (DEPs). The results showed that there were 4054 DEPs in total, among which the DEPs in the pedal mucus of the heat-resistance group and the control group were mainly immune-related heat shock proteins, calmodulin, Ig-like and fibronectin type III domain-containing proteins, histones and mucins, etc.; the DEPs related to growth metabolism included glutathione, growth factor receptor-bound protein, alanine aminotransferase, etc. Circadian entrainment signaling pathway and growth hormone synthesis, secretion and action signaling pathway was significantly enriched, cortisol synthesis and secretion signaling pathway was up-regulated in Haliotis discus hannai heat-resistance group. This study provided references for exploring the relationship between the mucus proteome difference in pedal mucus from different abalone populations sources. These results would contribute to further search for proteins related to immunity, growth and stress resistance, and provide theoretical basis for the development of high-quality germplasm resources of Pacific abalone.