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Ultrafast Dynamics in Flavocytochrome C by Using Transient Absorption and Femtosecond Fluorescence Lifetime
Krishna P Khakurel1, Gustavo Fuertes2, Aron Sipos3
1Extreme Light Infrastructure ERIC, Dolni Brezany CZ-25241, Czech Republic.
The Journal of Physical Chemistry. B
|April 8, 2025
Summary
Ultrafast spectroscopy reveals flavocytochrome c sulfide dehydrogenase (FCC) dynamics. Photoexcited states decay rapidly, with internal conversion and vibrational cooling occurring within picoseconds, primarily impacting the heme group.
Area of Science:
- Biochemistry
- Biophysics
- Spectroscopy
Background:
- Flavocytochrome c sulfide dehydrogenase (FCC) is crucial for sulfur metabolism in bacteria.
- Its catalytic properties are well-studied, but its ultrafast dynamics remain unclear.
Purpose of the Study:
- To investigate the early photophysical events in flavocytochrome c (FccAB) from *Thiocapsa roseopersicina*.
- To understand the ultrafast dynamics of heme and flavin chromophores upon excitation.
Main Methods:
- Ultrafast transient absorption spectroscopy
- Fluorescence spectroscopy
Main Results:
- Fluorescence kinetics indicate rapid nonradiative decay of photoexcited species within picoseconds.
- Transient absorption reveals two main dynamic processes: internal conversion (~400 fs) and vibrational cooling (~4 ps).
- These dynamics primarily affect the heme moiety of FccAB.
Conclusions:
- The study elucidates the initial ultrafast processes governing FccAB photophysics.
- Understanding these dynamics is key to comprehending FCC's role in sulfur metabolism.
