RBM24 regulates apoptosis rates by modulating global transcriptome profile in CAL27 cells

Area of Science:

• Molecular Biology
• Genomics
• Cancer Research

Background:

• RNA binding proteins (RBPs) are crucial regulators of gene expression after transcription.
• RBM24 is identified as a pivotal gene linked to prognosis in oral squamous cell carcinoma (OSCC).

Purpose of the Study:

• To investigate the binding targets and post-transcriptional regulatory functions of RBM24 in OSCC.
• To elucidate the role of RBM24 in OSCC progression and identify potential therapeutic targets.

Main Methods:

• Utilized iRIP-seq (individual-nucleotide resolution crosslinking immunoprecipitation followed by sequencing) to identify RBM24 binding targets.
• Employed RNA-seq (RNA sequencing) to analyze global gene expression changes in RBM24-overexpressing cells.
• Compared gene expression profiles between RBM24-overexpressing and control cell lines.

Main Results:

• RBM24 overexpression significantly altered gene expression, impacting pathways involved in apoptosis and immune inflammation.
• RBM24-regulated alternative splicing primarily affected genes in DNA damage repair and RNA metabolism.
• RBM24 was found to bind to both pre-mRNAs and long non-coding RNAs (lncRNAs).

Conclusions:

• RBM24 plays a significant role in the progression of oral squamous cell carcinoma.
• RBM24's regulation of gene expression and alternative splicing, including lncRNAs, highlights its importance in OSCC.
• Identifying RBM24's targets and functions may lead to novel clinical therapeutic strategies for OSCC.