Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors01:20

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

448
Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...
448
Formation of the Platelet Plug01:22

Formation of the Platelet Plug

3.6K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
3.6K
Structure and Function of Platelets01:18

Structure and Function of Platelets

940
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
940

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adaptive Mesh Refinement for Two-Phase Viscoelastic Fluid Mixture Models.

Computers & fluids·2026
Same author

A three-dimensional shear dependent continuum model of platelet aggregation under flow.

PLoS computational biology·2026
Same author

A scalable high-throughput platform for the discovery of intracellular and extracellular regulators of platelet migration.

Blood advances·2026
Same author

Platelet plug microstructure and flow modulate fibrin gelation dynamics: Insights from computational simulations.

ArXiv·2026
Same author

A novel G13-RAPGEF2-RAP1 signaling pathway critical for platelet adhesion and aggregation.

Blood·2026
Same author

Mechanisms of thrombin inhibition by protein S and the TFPIα-fVshort-protein S complex.

Biophysical journal·2026
Same journal

Heterogeneous binding of SARS-CoV2 fusion peptide on complex cellular membranes enhances its fusogenicity.

Biophysical journal·2026
Same journal

Tau protein differentially affects Piezo1 and Kir2.1 channels in brain capillary endothelial cells.

Biophysical journal·2026
Same journal

Emergent Intercellular Junction Stability during Cyclic Tissue Loading.

Biophysical journal·2026
Same journal

Enhanced-Sampling Simulations Reveal Distinct Intermediates in SARS-CoV-2 FSE Pseudoknot Interconversion.

Biophysical journal·2026
Same journal

Structure-based simulations of the full Flock House virus capsid reveal pathways and energetics of an infection-critical peptide externalization event.

Biophysical journal·2026
Same journal

Quantifying the Peripheral Surface Information Entropy from Conformational Ensembles of Globular Protein-Peptide Complexes.

Biophysical journal·2026
See all related articles

Related Experiment Video

Updated: May 23, 2025

Microfluidics in Assessing Platelet Function
06:47

Microfluidics in Assessing Platelet Function

Published on: November 8, 2024

738

Modeling platelet P2Y1/12 pathway to integrin activation.

Keshav B Patel1, Wolfgang Bergmeier2, Aaron L Fogelson3

  • 1Department of Mathematics, University of Utah, Salt Lake City, Utah.

Biophysical Journal
|April 9, 2025
PubMed
Summary
This summary is machine-generated.

This study models platelet aggregation, revealing how ADP activates integrin αIIbβ3. The findings clarify the roles of different pathways in RAP1 activation and predict cell behavior, aiding understanding of drug responses.

More Related Videos

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry
04:32

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry

Published on: June 5, 2019

7.6K
An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers
05:56

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers

Published on: November 8, 2024

240

Related Experiment Videos

Last Updated: May 23, 2025

Microfluidics in Assessing Platelet Function
06:47

Microfluidics in Assessing Platelet Function

Published on: November 8, 2024

738
A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry
04:32

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry

Published on: June 5, 2019

7.6K
An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers
05:56

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers

Published on: November 8, 2024

240

Area of Science:

  • Biochemistry
  • Cell Biology
  • Mathematical Biology

Background:

  • Platelet aggregation is crucial for hemostasis, involving integrin αIIbβ3 activation.
  • Adenosine diphosphate (ADP) triggers this via G protein-coupled receptors, impacting RAP1 signaling.

Purpose of the Study:

  • To quantify the contributions of distinct ADP-mediated pathways to RAP1-dependent integrin activation.
  • To predict cellular responses to varying agonist concentrations and genetic mutations.

Main Methods:

  • Development of a dynamical systems model of the signaling cascade up to RAP1 regulation.
  • Parameter estimation using flow cytometry data and validation with existing experimental results.

Main Results:

  • The model accurately reproduced known effects of impaired P2Y1 receptor desensitization and RASA3 expression.
  • It identified P2Y12 pathway components as critical regulators of integrin activation.

Conclusions:

  • The developed model enhances understanding of ADP-driven platelet activation and interindividual variability.
  • It provides insights into therapeutic P2Y12 inhibition and platelet aggregation dynamics.