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Tracking host-guest recognition in cells by a BODIPY·CB[7] complex.

Fengbo Liu¹, Haiqi Xiao¹, Quan Gao¹

¹Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518059, China. jin.geng@siat.ac.cn.

Chemical Communications (Cambridge, England)

|April 9, 2025

Summary

This summary is machine-generated.

Researchers explored host-guest binding between fluorescent BODIPY+ and cucurbit[7]uril for tracking lysosomes in cancer cells. They discovered that cucurbit[7]uril (CB[7]) causes BODIPY+ to deaggregate, enabling new cellular imaging applications.

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Area of Science:

Supramolecular Chemistry
Cell Biology
Biophysical Chemistry

Background:

Host-guest chemistry is crucial for understanding molecular recognition in biological systems.
Fluorescent probes are essential tools for visualizing cellular processes.
Lysosomes play vital roles in cellular function and disease.

Purpose of the Study:

To investigate the intracellular recognition between fluorescent BODIPY+ and cucurbit[7]uril (CB[7]).
To explore the application of this host-guest interaction for lysosome tracking in living cancer cells.
To characterize the effect of CB[7] complexation on the aggregation state of BODIPY+.

Main Methods:

Synthesis and characterization of fluorescent BODIPY+ derivatives.
Cell culture of living cancer cells.
Confocal fluorescence microscopy for lysosome imaging.
Spectroscopic techniques to study BODIPY+ aggregation and deaggregation.

Main Results:

Demonstrated specific intracellular recognition between BODIPY+ and CB[7] within living cancer cells.
Successfully utilized the BODIPY+/CB[7] complex for effective lysosome tracking.
Observed an unusual sequential deaggregation of spontaneously dimerized BODIPY+ upon complexation with CB[7].

Conclusions:

The BODIPY+/CB[7] system offers a novel approach for lysosome visualization in cellular environments.
The observed deaggregation phenomenon provides new insights into host-guest interactions involving fluorescent dyes.
This work opens avenues for developing advanced fluorescent probes for biomedical applications.