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Related Concept Videos

Imaging Studies III: Gastrointestinal Motility Studies and Virtual Colonoscopy01:26

Imaging Studies III: Gastrointestinal Motility Studies and Virtual Colonoscopy

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This lesson explores three gastrointestinal imaging techniques: radionuclide testing, colonic transit studies, and virtual colonoscopy.
Radionuclide Testing
Radionuclide testing is a sophisticated medical technique for assessing gastrointestinal motility. It focuses on gastric emptying and colonic transit time. Radioactive markers track the movement of food through the digestive system, providing insights into gastrointestinal disorders.
In gastric emptying studies, a meal's liquid and...
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Related Experiment Video

Updated: May 15, 2025

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
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3D visualization of human colon tissue using a modified CUBIC-based tissue-clearing technique.

Pavel Pavlov1, Andreas Kontny1, Neele Wagner1

  • 1Department of Anatomy and Cell Biology, Faculty of Medicine, Medical University Varna, Varna 9002, Bulgaria.

Journal of Biological Methods
|April 9, 2025
PubMed
Summary

Researchers adapted optical tissue clearing for routine labs to visualize 3D nerve structures in colon cancer. This method allows standard microscopes to image cleared tissues, aiding pathological analysis.

Keywords:
3D-imagingCUBICClear unobstructed brain/body imaging cocktailsColonColorectal cancerTissue clearing

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Area of Science:

  • Pathology
  • Neuroscience
  • Biotechnology

Background:

  • Colorectal cancer is a common disease requiring detailed pathological analysis.
  • Visualizing 3D structures like tumor nerves needs thick tissues and optical clearing.
  • Standard histology labs often lack advanced imaging for cleared samples.

Purpose of the Study:

  • Adapt established tissue clearing for routine histology labs.
  • Enable 3D visualization of nerve fibers in human colon and cancer tissues.
  • Simplify complex 3D imaging for broader accessibility.

Main Methods:

  • Modified the "clear unobstructed brain/body imaging cocktails" clearing method.
  • Applied the technique to approximately 10 mm³ human colon tissue samples.
  • Used standard epifluorescence microscopy and open-source ilastik software for analysis.

Main Results:

  • Achieved reliable immunofluorescent visualization of colonic nerve fibers (anti-β3-tubulin).
  • Enabled 3D reconstruction of nerve structures using standard epifluorescence microscopy.
  • Eliminated the need for specialized confocal microscopy for cleared samples.

Conclusions:

  • The protocol offers a valuable method for 3D visualization of neural structures.
  • Applicable to both normal and tumor-transformed colon tissue settings.
  • Facilitates research in complex 3D tissue architecture.