Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

C6-C7 Corpectomy for Clipping of Ruptured Anterior Radiculomedullary Artery Aneurysm: 2-Dimensional Operative Video.

Operative neurosurgery (Hagerstown, Md.)·2026
Same author

Expansile Duraplasty for Acute Spinal Cord Injury: A 2-Dimensional Operative Video.

Operative neurosurgery (Hagerstown, Md.)·2026
Same author

Procedures Leading to Iatrogenic Injury of the Sural Nerve.

Plastic and reconstructive surgery. Global open·2026
Same author

Comparative outcomes of syringopleural, syringosubarachnoid, and syringoperitoneal shunts for syringomyelia: a single-center retrospective cohort study.

Journal of neurosurgery·2026
Same author

The 6 + 1 model: Redefining the final year of neurosurgical training.

Clinical neurology and neurosurgery·2026
Same author

Propentofylline and Interleukin-4 Modulate Lesion-Associated Myeloid Responses and Improve Functional Recovery After Spinal Cord Injury.

Cells·2026

Related Experiment Video

Updated: May 15, 2025

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
12:27

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

4.5K

Human Schwann Cell-Derived Extracellular Vesicle Isolation, Bioactivity Assessment, and Omics Characterization.

Aisha Khan1,2, Julia Oliveira2, Yee-Shuan Lee1

  • 1Interdisciplinary Stem Cell Institute, Miller School of Medicine, University of Miami, Miami, FL, USA.

International Journal of Nanomedicine
|April 9, 2025
PubMed
Summary

This study presents a scalable method for isolating Schwann cell-derived extracellular vesicles (SCEVs) with consistent molecular profiles. These SCEVs show therapeutic potential for neurological repair and regeneration.

Keywords:
axon growthlipidomicmyelinationneuroprotectionregeneration

More Related Videos

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment
10:09

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Published on: June 2, 2020

6.8K
A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells
09:10

A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells

Published on: June 23, 2022

1.4K

Related Experiment Videos

Last Updated: May 15, 2025

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
12:27

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

4.5K
Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment
10:09

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Published on: June 2, 2020

6.8K
A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells
09:10

A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells

Published on: June 23, 2022

1.4K

Area of Science:

  • Neuroscience
  • Biotechnology
  • Cell Biology

Background:

  • Schwann cell-derived extracellular vesicles (SCEVs) show promise for treating spinal cord, peripheral nerve, and brain injuries.
  • Standardized and efficient isolation methods are crucial for clinical applications of SCEVs.

Purpose of the Study:

  • To develop a scalable, standardized, and efficient methodology for isolating SCEVs.
  • To characterize the molecular composition (proteomic, lipidomic, miRNA) of isolated SCEVs.
  • To confirm the therapeutic potential of SCEVs in promoting neurological repair.

Main Methods:

  • Human Schwann cells from nine donors were cultured and stimulated to produce SCEVs.
  • Extracellular vesicles (EVs) were isolated using sequential centrifugation.
  • EVs were characterized using mass spectrometry (proteomics), lipidomic analysis, and next-generation sequencing (miRNA).
  • In vitro assays assessed SCEV bioactivity for neurite outgrowth and cell survival.

Main Results:

  • The isolation method consistently yielded high amounts of SCEVs with reproducible molecular profiles.
  • SCEVs demonstrated bioactivity in promoting neurite outgrowth in vitro.
  • Proteomic analysis identified 136 proteins related to nervous system repair.
  • Lipidomic analysis revealed triacylglycerol and phosphatidylcholine, important for axon regeneration.
  • miRNA profiling identified 732 miRNAs, with top 30 potentially involved in neuroprotection and repair pathways.

Conclusions:

  • A robust framework for SCEV isolation and characterization was established.
  • The characterized SCEVs possess therapeutic potential for neurological applications.
  • The comprehensive dataset of SCEV molecular content will advance their use in treating neurological injuries and diseases.