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The Soft Agar Colony Formation Assay
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GART promotes the proliferation and migration of human non-small cell lung cancer cell lines A549 and H1299 by

Zhuo Chen1, Yu-Heng Ding2, Mei-Qi Zhao1

  • 1School of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.

Frontiers in Oncology
|April 9, 2025
PubMed
Summary
This summary is machine-generated.

Glycinamide ribonucleotide transformylase (GART) promotes lung adenocarcinoma (LUAD) by activating the PAICS-Akt-β-catenin pathway. Targeting GART may offer a new therapeutic strategy for non-small cell lung cancer (NSCLC).

Keywords:
GARTPAICScell migrationcell proliferationlung cancer

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Area of Science:

  • Molecular Oncology
  • Proteomics
  • Cancer Therapeutics

Background:

  • Lung adenocarcinoma (LUAD) is a major subtype of non-small cell lung cancer (NSCLC), with underlying molecular mechanisms requiring further elucidation.
  • Understanding the molecular drivers of LUAD is crucial for developing effective therapeutic strategies.

Purpose of the Study:

  • To identify key molecular players and pathways involved in LUAD pathogenesis through proteomic analysis.
  • To investigate the functional role and prognostic significance of Glycinamide ribonucleotide transformylase (GART) in LUAD.
  • To explore the therapeutic potential of targeting GART in NSCLC.

Main Methods:

  • Proteomic analysis of 20 primary lung cancer (LC) tumors and paired normal tissues.
  • Bioinformatics analysis to identify differentially expressed proteins and associated pathways.
  • Validation of GART expression and its prognostic value in LUAD using public databases (Ualcan).
  • In vitro (cell lines) and in vivo (xenograft model) functional assays to assess GART's role in cell proliferation and migration.
  • qRT-PCR and Western blot to analyze the PAICS-Akt-β-catenin pathway activation.

Main Results:

  • Proteomic analysis identified 263 upregulated and 194 downregulated proteins in LC tissues, associated with apoptosis, cell adhesion, PI3K-Akt, purine metabolism, and Wnt signaling.
  • Hub proteins, including GART, were significantly overexpressed in LUAD tissues, with GART overexpression correlating with poor prognosis.
  • GART knockdown inhibited LC cell proliferation and migration in vitro and in vivo, and suppressed the PAICS-Akt-β-catenin pathway.

Conclusions:

  • GART exhibits a tumor-promoting function in lung cancer by regulating the PAICS-Akt-β-catenin signaling axis.
  • GART represents a potential therapeutic target for non-small cell lung cancer (NSCLC).