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Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...

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Related Experiment Video

Updated: May 15, 2025

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Published on: January 14, 2021

Applications of spatial transcriptomics in studying spermatogenesis.

Qianlan Xu^1,2, Haiqi Chen^1,2

¹Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, Texas, USA.

|April 9, 2025

Summary

This summary is machine-generated.

Spatial transcriptomics reveals gene expression and cell interactions within the native testicular tissue. This technology aids in understanding male infertility causes and developing new treatments.

Keywords:

cell‒cell communications infertility spatial transcriptomics spermatogenesis

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Last Updated: May 15, 2025

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Published on: January 14, 2021

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Published on: February 6, 2018

Area of Science:

Reproductive Biology
Genomics
Spatial Transcriptomics

Background:

Spermatogenesis is a complex cellular differentiation process crucial for male fertility.
Traditional methods like single-cell RNA sequencing lose vital tissue context, hindering understanding of spermatogenesis regulation and infertility causes.
Advancements in spatial transcriptomics offer a solution by enabling molecular analysis within the native tissue environment.

Purpose of the Study:

To review the application of spatial transcriptomics in studying the mammalian testis.
To highlight how this technology elucidates gene expression, cellular neighborhoods, and cell-cell communications in situ.
To explore its potential in understanding male infertility and guiding therapeutic strategies.

Main Methods:

Review of studies utilizing spatial transcriptomics in mammalian testis research.
Analysis of spatial transcriptomics data to identify spatially variable genes.
Characterization of cellular neighborhoods and intercellular communication networks within the testicular tissue.

Main Results:

Spatial transcriptomics successfully identifies spatially defined gene expression patterns in the testis.
It enables detailed characterization of cellular composition and spatial relationships.
The technology can detect molecular alterations associated with pathological conditions in the testis.

Conclusions:

Spatial transcriptomics provides unprecedented insights into the spatial organization of the mammalian testis.
It is a powerful tool for dissecting the molecular mechanisms underlying spermatogenesis and male infertility.
Integration with other spatially resolved omics assays promises to accelerate the discovery of novel therapeutic targets for infertility.