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Updated: May 15, 2025
07:58
A Polyaniline-based Sensor of Nucleic Acids
Published on: November 1, 2016
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Cationic and anionic phenothiazine derivatives: electrochemical behavior and application in DNA sensor development.
Anastasia N Malanina1, Yury I Kuzin1, Pavel L Padnya1
1A.M. Butlerov Institute of Chemistry, Kazan Federal University, 18, Kremlevskaya Street, Kazan 420008, Russian Federation.
The Analyst
|April 9, 2025
Summary
Novel phenothiazine derivatives were explored for modifying electrodes in voltammetric sensors. A DNA sensor developed using these materials achieved a low limit of detection for doxorubicin, advancing point-of-care diagnostics.
Area of Science:
- Electrochemistry
- Materials Science
- Analytical Chemistry
Background:
- The demand for personalized medicine and point-of-care testing necessitates enhanced sensor performance.
- Novel electrode materials are crucial for improving voltammetric sensor capabilities.
Purpose of the Study:
- To electrochemically investigate two phenothiazine derivatives with different functional groups.
- To develop and characterize modified electrodes for sensor applications.
- To create a DNA sensor for doxorubicin detection.
Main Methods:
- Cyclic voltammetry
- Electrochemical impedance spectroscopy
- Quartz crystal microbalance
- Scanning electron microscopy
- Electrode modification via electropolymerization and consecutive electrodeposition
Main Results:
- Phenothiazine derivatives exhibited mutual influence on voltammetric signals.
- Consecutive electrodeposition yielded more uniform and thicker redox-active films compared to electropolymerization.
- A DNA sensor demonstrated a linear response to doxorubicin concentration over two ranges (0.1 fM–1 nM and 1 nM–1 μM).
- The sensor achieved a limit of detection of 0.6 fM for doxorubicin.
Conclusions:
- Phenothiazine derivatives are promising materials for electrochemical sensor development.
- The developed DNA sensor shows high sensitivity and potential for point-of-care applications.
- Consecutive electrodeposition is an effective method for creating high-performance sensor electrodes.
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