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Published on: March 4, 2018
Sperm Cryopreservation in Boars
Inmaculada Parrilla1, María A Gil2, Cristina Cuello2
1Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research, Biomedical Research Institute of Murcia (IMIB-Pascual Parrilla), University of Murcia, Murcia, Spain. parrilla@um.es.
Abstract:
Cryopreservation is the procedure that allows preserving cells, including gametes, for long time periods while maintaining their functionality, through the use of cryogenic temperature. Currently, sperm cryopreservation is a crucial-assisted reproductive biotechnology in pigs since it is the only effective and efficient method for long-term preservation of boar fertility. Here, we describe a two-step cryopreservation protocol for boar sperm using lactose-egg yolk and glycerol-based extenders.
