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Updated: May 8, 2025

Fluorimetric Techniques for the Assessment of Sperm Membranes
Published on: November 28, 2018
Detection of Sperm Membrane Proteins by Immunocytochemistry, Scanning and Transmission Electron Microscopy, and
1Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, Girona, Spain. elisabeth.pinart@udg.edu.
Abstract:
Western blotting in combination with scanning and transmission electron microscopy provide accurate and complementary information on the presence and localization of proteins in the plasma membrane, as well as the structural and ultrastructural features of sperm cells. The present chapter describes the procedures to detect the presence of membrane proteins by Western blotting and to localize them in the plasma membrane of sperm cells.
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Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

