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Detection and Quantification of mRNA in Mammalian Spermatozoa

Won-Ki Pang1, Myung-Geol Pang2

  • 1Department of Animal Science & Technology and BET Research Institute, Chung-Ang University, Anseong, Gyeonggi-do, Republic of Korea.

Methods in Molecular Biology (Clifton, N.J.)
|April 9, 2025
PubMed

Keywords:
Guanidine-isothiocyanate lysis bufferRT-qPCRSilica-based columnSpermatozoamessenger RNA

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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...