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Updated: May 20, 2025
Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas
Published on: May 31, 2012
Human proteome distribution atlas for tissue-specific plasma proteome dynamics.
Erik Malmström1, Lars Malmström2, Simon Hauri3
1Division of infection medicine, Department of Clinical Sciences Lund, Lund University, 22184 Lund, Sweden; Emergency Medicine, Department of Clinical Sciences Lund, Faculty of Medicine, Lund University, Skåne University Hospital, Lund, Sweden; Department of Emergency medicine and Internal medicine, Emergency department, Skåne University Hospital, Lund, Sweden.
Researchers developed a mass-spectrometry proteomics strategy to identify the organ origin of plasma proteins. This tool quantifies organ-specific proteins in plasma, aiding disease research.
Area of Science:
- Proteomics
- Systems Biology
- Biochemistry
Background:
- The plasma proteome, crucial for health, is dynamically regulated by protein exchange with organs and cells.
- Understanding the contribution of different tissues to plasma protein levels is vital in both healthy and diseased states.
Purpose of the Study:
- To develop and validate a mass-spectrometry-based proteomics strategy for inferring the tissue origin of plasma proteins.
- To quantify the impact of various organs and cell types on the plasma proteome in health and disease.
Main Methods:
- Construction of a comprehensive human proteome atlas integrating data from 18 vascularized organs and 8 blood cell types.
- Integration of the atlas with existing RNA and protein datasets to establish proteome-wide protein-organ associations.
- Application of the strategy to analyze organ-specific protein changes in patient cohorts with sepsis, pancreatitis, and myocardial injury.
Main Results:
- Successfully created a human proteome atlas enabling objective definition of protein-organ associations.
- Demonstrated the ability to reproducibly quantify organ-specific proteins in human plasma.
- Identified disease-specific quantitative changes in organ-enriched protein panels across multiple patient cohorts.
Conclusions:
- The developed proteomics strategy provides a powerful resource for determining the tissue origin of plasma proteins.
- This approach enables quantitative analysis of organ-specific plasma proteins, advancing understanding of disease pathophysiology.
- The strategy is extensible to various diseases, offering new avenues for investigating plasma proteome dynamics.
