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Updated: May 12, 2026

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
Published on: January 7, 2019
Advances in multi-omics technologies for identifying metabolic engineering targets and improving lipid production in
Chun-Xiao Yan1, Shuai Zhang2, Lu-Wei Xu2
1State Key Laboratory of Microbial Technology, Nanjing Normal University, Nanjing, China; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 2 Xuelin Road, Qixia District, Nanjing, People's Republic of China.
Abstract:
Polyunsaturated fatty acids (PUFAs), such as γ-linolenic acid, arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid, are highly valued in the global market due to their physiological effects and health benefits. Concerns related to overfishing and marine ecosystem degradation have driven interest in microalgal lipids as a sustainable and eco-friendly alternative for PUFA production. Despite some success in commercializing microalgal lipid products, they still fail to meet global demand. Advances in high-throughput omics technologies, including genomics, transcriptomics, proteomics, and metabolomics, have deepened the understanding of lipid biosynthesis in microalgae. This review explores the potential of multi-omics approaches to elucidate PUFA biosynthesis pathways, identify key regulatory genes, and optimize metabolic engineering strategies for enhanced lipid production. Additionally, this review discusses how multi-omics technologies address challenges in large-scale cultivation, promoting the industrialization of microalgal lipid productions. These insights provide a foundation for improving microalgal PUFA yields to meet growing global demand.
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