Related Experiment Video
Updated: May 15, 2025
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
Published on: November 30, 2016
Analysis of miRNA Expression in Patients With NSAID-Exacerbated Respiratory Disease
Adrian Gajewski1, Adrian Bekier1, Karolina Frachowicz-Guereirro1
1Department of Immunology and Allergy, Medical University of Lodz, Lodz, Poland.
Purpose:
Non-steroidal anti-inflammatory drug-exacerbated respiratory disease (N-ERD) is a phenotype of bronchial asthma that is characterized by a severe course and the presence of chronic rhinosinusitis (CRS) with nasal polyps. MicroRNAs (miRNAs) belong to a family of small, non-coding RNAs whose primary function is to regulate gene transcription. The aim of this study was to determine the miRNA profile and to validate selected miRNAs in biological material from the upper respiratory tract collected with a minimally-invasive method in patients with N-ERD.
Methods:
The miRNA profile was assessed in subjects with N-ERD, CRS, and allergic asthma (AA), as well as healthy controls (HCs), using microarray technique. Following this, 6 miRNAs were validated using reverse transcription polymerase chain reaction in 77 subjects.
Results:
The profiling identified 23 miRNAs whose expression significantly differed between patients with N-ERD and HCs. Based on these results, 6 miRNAs were selected for further validation. It was found that patients with N-ERD had significantly different expressions of miR-34a-5p and miR-22-5p compared to those with AA. In the whole study group, significant correlations were found between miR-7d-3p/miR-34a-5p/miR-22-5p and the presence of blood eosinophilia (r = 0.25, r = 0.28 and r = 0.26, for all P < 0.05). Forced expiratory volume in 1 second/forced vital capacity was correlated with miR-149a-5p expression (r = 0.27, P < 0.05).
Conclusions:
The results indicate that the miRNA profile in nasal mucosal lining fluid of patients with N-ERD differs from patients with AA, CRS, and compared to HCs. Some of the miRNAs selected on the basis of profiling may be involved in the regulation of eosinophilic inflammation in the respiratory tract. Our findings suggest that specific miRNAs may be considered as potential biomarkers of N-ERD.
Insights
MicroRNA (miRNA) profiles in nasal lining fluid differ in patients with non-steroidal anti-inflammatory drug-exacerbated respiratory disease (N-ERD). Some miRNAs may regulate eosinophilic inflammation, suggesting potential N-ERD biomarkers.
Area of Science:
- Respiratory Medicine
- Molecular Biology
- Genetics
Background:
- Non-steroidal anti-inflammatory drug-exacerbated respiratory disease (N-ERD) is a severe asthma phenotype.
- N-ERD is characterized by chronic rhinosinusitis (CRS) with nasal polyps.
- MicroRNAs (miRNAs) are small non-coding RNAs regulating gene expression.
Purpose of the Study:
- To determine the miRNA profile in the upper respiratory tract of N-ERD patients.
- To validate selected miRNAs using minimally-invasive sampling.
- To compare miRNA profiles between N-ERD, CRS, allergic asthma (AA), and healthy controls (HCs).
Main Methods:
- Microarray technique to assess miRNA profiles in N-ERD, CRS, AA, and HCs.
- Reverse transcription polymerase chain reaction (RT-PCR) for validating 6 selected miRNAs.
- Study included 77 subjects.
Main Results:
- 23 differentially expressed miRNAs identified between N-ERD and HCs.
- N-ERD patients showed significantly different expressions of miR-34a-5p and miR-22-5p compared to AA patients.
- Correlations found between specific miRNAs (miR-7d-3p, miR-34a-5p, miR-22-5p) and blood eosinophilia.
- miR-149a-5p expression correlated with lung function (FEV1/FVC).
Conclusions:
- miRNA profiles in nasal mucosal lining fluid distinguish N-ERD patients from AA, CRS, and HCs.
- Selected miRNAs may play a role in regulating eosinophilic airway inflammation.
- Specific miRNAs show potential as diagnostic biomarkers for N-ERD.
