Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

EV-Finder: Direct Detection of Extracellular Vesicle-Associated Proteins by Proximity Extension Assay for Multi-Cancer Screening.

International journal of molecular sciences·2026
Same author

A Dual-Imaging Fluorescent and Iodinated Thermosensitive Hydrogel for Image-Guided Surgery of Pulmonary Nodules.

ACS applied bio materials·2026
Same author

Supramolecular Chiral Assembly of Open-Shell Quinoids With Chiral Additives and Their Spin-Dependent Transport in Magneto Field-Effect Transistors.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

WWP2 ubiquitin ligase promotes colorectal cancer progression by targeting p53 for degradation: an experimental study.

Annals of surgical treatment and research·2026
Same author

Surfactant-Triggered Signal Enhancement for Lateral Flow Immunoassay.

ACS sensors·2026
Same author

<i>Schisandra chinensis</i> Pomace Attenuates Scopolamine-Induced Cholinergic Dysfunction Associated with Changes in BDNF and JNK Signaling.

Current issues in molecular biology·2026
Same journal

Chlorinated VSLSs Surpass HCFCs in CFC-11-Equivalent Emissions for Ozone Layer Depletion in China.

Nature communications·2026
Same journal

Author Correction: Charge transfer in triphenylamine-tetrazine covalent organic frameworks for solar-driven hydrogen peroxide production.

Nature communications·2026
Same journal

Vegetation browning patterns under compound soil and atmospheric dryness in northern permafrost ecosystems.

Nature communications·2026
Same journal

Voltage imaging of CA1 pyramidal cells and SST+ interneurons reveals stability and plasticity mechanisms of spatial firing.

Nature communications·2026
Same journal

Radical-omics reveals the hydrogen-abstraction pathway of isoprene oxidation.

Nature communications·2026
Same journal

Toughening elastomer via sequentially activated multi-pathway energy dissipation.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: May 15, 2025

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.1K

Lateral flow immunoassay using plasmonic scattering.

Bobin Lee1, Byungho Park1, Daeun Kim1,2

  • 1Advanced Photonics Research Institute (APRI), Gwangju Institute of Science and Technology (GIST), Gwangju, 61005, Republic of Korea.

Nature Communications
|April 9, 2025
PubMed
Summary
This summary is machine-generated.

A novel plasmonic scattering lateral flow immunoassay (LFIA) enhances sensitivity for point-of-care diagnostics. This improved LFIA achieves near PCR-level detection for influenza A without complex equipment.

More Related Videos

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

7.5K
Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.0K

Related Experiment Videos

Last Updated: May 15, 2025

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.1K
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

7.5K
Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.0K

Area of Science:

  • Nanotechnology and Biosensing
  • Point-of-Care Diagnostics
  • Immunological Assays

Background:

  • Lateral flow immunoassays (LFIA) are widely used for point-of-care (POC) testing due to their speed, affordability, and ease of use.
  • A significant limitation of traditional LFIA is its lower sensitivity compared to laboratory methods like PCR, hindering its application in early disease detection.
  • Enhancing LFIA sensitivity without compromising its practical advantages remains a critical challenge in diagnostic development.

Purpose of the Study:

  • To develop a highly sensitive LFIA by utilizing plasmonic scattering principles.
  • To enable naked-eye detection of analytes at concentrations comparable to PCR.
  • To improve the diagnostic performance of LFIA for infectious diseases like influenza A.

Main Methods:

  • A modified LFIA was designed using a transparent nitrocellulose membrane and a light-absorbing backing card.
  • This configuration minimizes background noise and enhances the detection of plasmonic scattering signals from gold nanoparticles.
  • The assay was validated using influenza A detection and compared against commercial LFIAs and PCR sensitivity benchmarks.

Main Results:

  • The plasmonic scattering LFIA demonstrated a 2600-4400 times lower detection limit for influenza A compared to commercial LFIAs.
  • Clinical validation showed 90% sensitivity, approaching the level of PCR-based diagnostics.
  • Commercial LFIAs exhibited significantly lower sensitivity, ranging from 23-30% in the same clinical validation.

Conclusions:

  • The proposed plasmonic scattering LFIA significantly overcomes the sensitivity limitations of conventional LFIAs.
  • This technology offers a promising platform for high-sensitivity, naked-eye, point-of-care diagnostics.
  • The advancement holds potential for revolutionizing infectious disease detection and other POC applications.